Thermo Fisher Scientific NOX2/gp91phox Polyclonal Antibody
다양한 종(인간, 마우스, 랫, 토끼, 치킨)에 반응하는 Rabbit Polyclonal 항체. Western blot, IHC, ICC/IF, Flow cytometry, ELISA 등 다양한 응용에 적합. KLH 결합 합성 펩타이드(aa 501-570)로부터 유래. Protein A 정제, 1 mg/mL 농도, -20°C 보관.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:300 |
| Immunohistochemistry (Paraffin) (IHC-P) | 1:200 |
| Immunohistochemistry (Frozen) (IHC-F) | 1:100–1:500 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
| Flow Cytometry (Flow) | 1:20–1:100 |
| ELISA | 1:500–1:1,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Chicken, Human, Mouse, Rabbit, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | KLH-conjugated synthetic peptide derived from human NOX2/gp91phox (amino acids 501–570) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | 0.01M TBS, pH 7.4, with 50% glycerol, 1% BSA |
| Contains | 0.02% ProClin 300 |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
Mox1 and the glycoprotein gp91-phox are essential components of the NADPH oxidase complex, which generates superoxide in phagocytes, neuroepithelial bodies, vascular smooth muscle cells, and endothelial cells. This complex includes membrane-bound flavocytochrome subunits (gp91-phox and p22-phox) and cytosolic proteins (p47-phox, p67-phox). The gp91-phox and p22-phox subunits also serve as surface O₂ sensors that initiate cellular signaling in response to hypoxia.
Mox1 and gp91 share identical C-terminal sequence identity but have distinct expression patterns: gp91-phox is expressed in eosinophils, neutrophils, monocytes, and B-lymphocytes, while Mox1 is mainly found in the colon, with low expression in the uterus and prostate.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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