
Thermo Fisher Scientific BAD Polyclonal Antibody
BAD 단백질을 인식하는 Rabbit Polyclonal 항체로, Western blot, ICC/IF, ELISA에 사용 가능. 인간, 마우스, 랫트 반응성. PBS/glycerol 버퍼에 보관되며, 세포사멸 연구에 적합. -20°C에서 보관 권장.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunocytochemistry (ICC/IF) | 1:10–1:100 |
| ELISA | 1 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthetic peptide corresponding to a sequence within amino acids 50–150 of human BAD (NP_004313.1) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 3.10 mg/mL |
| Purification | Affinity Chromatography |
| Storage Buffer | PBS, pH 7.3, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2913976 |
Product Specific Information
- Positive test controls: HeLa, Jurkat
- Subcellular localization: Cytoplasm, Mitochondrion outer membrane
Immunogen sequence:
HQQEQPTSSS HHGGAGAVEI RSRHSSYPAG TEDDEGMGEE PSPFRGRSRS APPNLWAAQR YGRELRRMSD EFVDSFKKGL PRPKSAGTAT QMRQSSSWTR V
Target Information
The protein encoded by BAD is a member of the BCL-2 family, known to regulate programmed cell death. BAD promotes apoptosis by forming heterodimers with BCL-xL and BCL-2, counteracting their anti-apoptotic functions. Its activity is regulated by phosphorylation through kinases such as AKT and MAP kinase, and by dephosphorylation via calcineurin. Alternative splicing results in two transcript variants encoding the same isoform.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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