Thermo Fisher Scientific Easy-DNA gDNA Purification Kit

Invitrogen™ Easy-DNA™ gDNA Purification Kit

The Easy-DNA™ Kit provides a fast and easy method for isolating high-quality, high-molecular-weight genomic DNA (gDNA) from a wide range of cells and tissue types. Sample sizes can range from a single hair follicle up to 1 gram of mammalian tissue. Tested samples include mouse tails, plant leaves, yeast, and E. coli cells. The resulting DNA typically ranges from 100 kb to 200 kb, suitable for PCR, DNA hybridization, genomic DNA library construction, and other molecular biology applications.

주요 특징

• Reliability — High-quality genomic DNA from diverse sample types (cells, tissue, plant, yeast, E. coli, blood, hair follicles)
• Simplicity — Only 4 steps required; typically less than 90 minutes for isolation
• Versatility — Optimized protocols for various cell and tissue types included

간단한 워크플로우

Small-scale gDNA preparations are performed in microfuge tubes. Samples are mixed with Lysis Solution and incubated at 65°C for 10 minutes. After chloroform extraction (not included), precipitation, and reconstitution of the DNA pellet, the preparation is complete.
The kit includes:

• Protein degradation reagent (for high-protein samples such as connective tissue)
• Mussel glycogen (co-precipitant for dilute DNA samples)
• RNase solution

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