Invitrogen™

Easy-DNA™ gDNA Purification Kit

The Easy-DNA™ Kit is a fast and easy way to isolate high-quality, high-molecular weight genomic DNA (gDNA) from a wide자세히 알아보기

The Easy-DNA™ Kit is a fast and easy way to isolate high-quality, high-molecular weight genomic DNA (gDNA) from a wide range of cells and tissue types. Sample sizes can range from a single hair follicle up to 1 gram of mammalian tissue. Other samples tested include mouse tails, plant leaves, yeast, and E. coli cells. These samples yield high-quality DNA with an average size between 100 kb and 200 kb, which is suitable for PCR, DNA hybridization, genomic DNA library construction, and other applications. Advantages of the Easy-DNA™ Kit include:

• Reliability—high-quality genomic DNA from large or small samples of cells, tissue, plant, yeast, E. coli, blood, and even hair follicles
• Simplicity—for most sample types, only 4 steps, typically requiring less than 90 minutes are needed to isolate high-quality DNA
• Versatility—one kit is supported by optimized protocols for a variety of cell and tissue types

Simple workflow, quality results
Optimized protocols for a variety of cell and tissue types are included in the instruction manual supplied with the kit. Generally, small-scale gDNA preparations are performed in microfuge tubes, in which samples are mixed with Lysis Solution and incubated at 65°C for 10 minutes. After extraction with chloroform (not included in kit), precipitation, and reconstitution of the DNA pellet, the preparation is complete. The kit contains a protein degradation reagent (for use with high-protein–containing samples such as connective tissue), mussel glycogen for use as a co-precipitant for dilute DNA samples, and an RNase solution.

사양

Isolation TechnologyOrganic Extraction

샘플 종류Bacteria, Cells, Hair Follicles, Mouse Tails, Plant, Tissue, Virus, Whole Blood, Yeast

용리량10 to 150 μL

최종 제품 유형gDNA

용도(애플리케이션)PCR, Southern blotting, sequencing, nucleic acid labeling, hybridization

고처리량 호환성Not High-throughput Compatible (Manual)

수량1 Kit

배송 조건Room Temperature

테스트 시간Up to 90 min.

수율≤850 μg

시작 물질 양Bacteria: ≤109 cells
Cells: ≤108
Hair Follicles: ≤5
Mouse Tails: 1 cm
Plant: ≤50 mg
Tissue: ≤1 g
Virus: ≤750 μL
Whole Blood: ≤2 mL
Yeast: ≤10 mL

Unit SizeEach