
Thermo Fisher Scientific RPA2 Monoclonal Antibody (OTI9A1), TrueMAB
RPA2 단백질을 인식하는 Mouse IgG1 모노클로날 항체로, Western blot, IHC, ICC, Flow cytometry, IP 등에 적합합니다. 인간 시료 반응성이 있으며, 동결건조 형태로 제공되어 안정적 보관이 가능합니다. 연구용 DNA 복구 및 복제 관련 실험에 활용됩니다.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50 |
| Immunocytochemistry (ICC/IF) | 1:100 |
| Flow Cytometry (Flow) | 1:100 |
| Immunoprecipitation (IP) | 2 µg/500 µL |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI9A1 |
| Immunogen | Full-length human recombinant RPA2 protein produced in HEK293T cells |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, avoid freeze/thaw cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL of distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).
Target Information
RPA2 is required for DNA recombination, repair, and replication. Its activity is mediated through single-stranded DNA binding and protein interactions, and it is essential for efficient recruitment of the DNA double-strand break repair factor RAD51 to chromatin in response to DNA damage.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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