Thermo Fisher Scientific gamma Actin Polyclonal Antibody
gamma Actin 단백질을 인식하는 Rabbit Polyclonal 항체로, Western blot, IHC, ICC/IF, Flow cytometry에 적합. 다양한 종에 교차 반응성이 예측되며, 세포골격 연구에 유용. 액상 형태로 제공되며 2 mg/mL 농도, 4°C 보관 권장.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:1,000 | - |
| Immunohistochemistry (Frozen) (IHC (F)) | - | 1 publication |
| Immunocytochemistry (ICC/IF) | 1:10–1:50 | 1 publication |
| Flow Cytometry (Flow) | 1:10–1:50 | - |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Published Species | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | KLH conjugated synthetic peptide between 188–215 amino acids from the central region of human ACTG1 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 2 mg/mL |
| Purification | Size-exclusion - Dialysis, Ammonium sulfate precipitation |
| Storage Buffer | PBS, pH 7.4 |
| Contains | 0.09% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2223522 |
Product Specific Information
This antibody is predicted to react with bovine, chicken, canine, equine, hamster, mouse, porcine, non-human primate, rat, rabbit, Xenopus, yeast, and zebrafish based on sequence homology.
Target Information
Actins are highly conserved proteins involved in various types of cell motility and cytoskeleton maintenance.
Three main groups of actin isoforms exist in vertebrates: alpha, beta, and gamma.
- Alpha actins: Found in muscle tissues, major components of the contractile apparatus.
- Beta and gamma actins: Co-exist in most cell types as cytoskeletal components and mediators of internal cell motility.
Actin gamma 1 (ACTG1) is a cytoplasmic actin found in all cell types.
Mutations in this gene are associated with DFNA20/26 (autosomal dominant non-syndromic progressive hearing loss) and Baraitser-Winter syndrome.
Alternative splicing results in multiple transcript variants.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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