Thermo Fisher Scientific CD45 Monoclonal Antibody (HI30), Brilliant Violet 711, eBioscience

Thermo Fisher Scientific CD45 Monoclonal Antibody (HI30), Brilliant Violet 711, eBioscience

Applications

• Flow Cytometry (Flow)

Tested Dilution: 5 µL (0.125 µg)/test

Product Specifications

Additional Formats

• Unconjugated
• Alexa Fluor 532, 561, 647, 660
• APC, APC-eFluor 780
• Biotin
• Brilliant UV series (395–805)
• Brilliant Violet series (421–650)
• eFluor 450, 506
• FITC
• NovaFluor Blue, Red, Yellow series
• PE, PE-Cyanine5, PE-Cyanine5.5, PE-Cyanine7
• PE-eFluor 610
• PerCP-Cyanine5.5, PerCP-eFluor 710
• Request custom conjugation available

Product Specific Information

The HI30 monoclonal antibody reacts with all isoforms of human CD45 (Leukocyte Common Antigen, LCA). CD45 is expressed by all hematopoietic cells excluding erythrocytes and platelets. It possesses tyrosine phosphatase activity and plays a key role in lymphocyte activation.

• Applications Reported: Flow cytometric analysis
• Applications Tested: Pre-diluted and tested on human peripheral blood cells
  • Recommended use: 5 µL (0.125 µg) per test (100 µL final volume)
  • Cell count range: 10⁵–10⁸ cells/test

Brilliant Violet™ 711 (BV711)

• Tandem dye emitting at 713 nm, excitable by 405 nm laser
• Ensure cytometer compatibility before use

Buffer Recommendation:
When using multiple polymer dye-conjugated antibodies, employ:

• Super Bright Complete Staining Buffer (Product # SB-4401-42)
• Brilliant Stain Buffer™ (Product # 00-4409-75)

Light Sensitivity:
Protect vial and stained samples from light to prevent photo-induced oxidation.

Fixation Compatibility:

• Compatible with IC Fixation Buffer (Product # 00-8222-49) or 1-step Fix/Lyse Solution (Product # 00-5333-54)
• Store samples up to 3 days at 4°C, in dark
• Not compatible with methanol-based fixation

Excitation: 407 nm
Emission: 713 nm
Laser: Violet Laser

BRILLIANT VIOLET™ is a trademark or registered trademark of Becton, Dickinson and Company or its affiliates, used under license. Powered by Sirigen™.

Target Information

CD45 (LCA) is a receptor-type protein tyrosine phosphatase expressed on all nucleated hematopoietic cells, constituting ~10% of lymphocyte surface proteins. It is absent from non-hematopoietic tissues and plays a crucial role in lymphocyte development and antigen signaling.

• Regulates Src-family kinases and cytokine receptor signaling
• Exists in multiple isoforms via alternative splicing
• Isoform expression varies by cell type and physiological state
• Influences integrin-mediated signaling, apoptosis, and immune regulation

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

제품 이미지