Thermo Fisher Scientific eIF2 beta Polyclonal Antibody
Rabbit polyclonal antibody targeting eIF2 beta for use in WB, IHC, and ICC/IF. Reacts with human and predicted cross-reactivity in multiple species. Supplied unconjugated, 1 mg/mL in PBS with BSA and glycerol. Ideal for protein synthesis studies.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:5,000–1:20,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:100–1:1,000 |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant fragment corresponding to a region within amino acids 1–216 of Human EIF2 beta |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7, with 1% BSA, 20% glycerol |
| Contains | 0.01% thimerosal |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2547767 |
Product Specific Information
- Recommended positive controls: A549, HeLa
- Predicted reactivity: Mouse (89%), Rat (92%), Rabbit (96%), Chicken (88%), Rhesus Monkey (99%), Chimpanzee (99%), Bovine (93%)
- Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
Eukaryotic translation initiation factor 2 (EIF-2) functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA and binding to a 40S ribosomal subunit. EIF-2 is composed of three subunits—alpha, beta, and gamma—with the protein encoded by this gene representing the beta subunit. The beta subunit catalyzes the exchange of GDP for GTP, recycling the EIF-2 complex for another round of initiation. Multiple transcript variants encoding different isoforms have been found for this gene.
⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm.
For more information, visit www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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