Thermo Fisher Scientific RNF3 Polyclonal Antibody

Applications

• Immunocytochemistry (ICC/IF)
  Tested Dilution: 0.25–2 µg/mL

Product Specifications

Product Specific Information

Immunogen sequence:
EEKPEEDNDYH RSDEQVSICL ECNSSKLRGL KRKWIRCSAQ ATVLHLKKFI

Highest antigen sequence identity to the following orthologs:

• Mouse: 98%
• Rat: 98%

Target Information

Phosphorylase kinase is a hexadecameric enzyme composed of four copies of four subunits encoded by PHKA, PHKB, PHKG, and PH genes. This serine/threonine-specific kinase converts glycogen phosphorylase b to glycogen phosphorylase a, leading to the release of glucose-1-phosphate from glycogen.

PHKB (Phosphorylase b kinase regulatory subunit beta) is a 1093-amino-acid subunit that, along with PHKA, has regulatory functions controlled by phosphorylation. Defects in the PHKB gene cause glycogen storage disease type 9B (phosphorylase kinase deficiency of liver and muscle), characterized by mild hepatomegaly, slightly elevated transaminase and plasma lipids, no clinical muscle involvement, and gradual improvement with age.

Four isoforms of PHKB are produced through alternative splicing events.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.