Thermo Fisher Scientific Importin 11 Polyclonal Antibody
Human Importin 11 단백질을 인식하는 Rabbit Polyclonal 항체로, Western blot, IHC, ICC/IF에 적합합니다. 항원 친화 크로마토그래피로 정제되었으며, PBS/glycerol buffer에 보존됩니다. 핵 단백질 수송 연구용으로 적합한 고품질 연구용 항체입니다.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.04–0.4 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:500–1:1,000 |
| Immunocytochemistry (ICC/IF) | 0.25–2 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant protein corresponding to Human Importin 11. Recombinant protein control fragment (Product #RP-104137) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 40% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2642713 |
Product Specific Information
Immunogen sequence:
QLLGNMIEMW VDRMDNITQP ERRKLSALAL LSLLPSDNSV IQDKFCGIIN ISVEGLHDVM TEDPETGTYK DCMLMSHLEE PKVTEDEEPP TEQD
Sequence identity (orthologs):
- Mouse: 94%
- Rat: 77%
Target Information
IPO11 functions in nuclear protein import as a nuclear transport receptor. It serves as a receptor for nuclear localization signals (NLS) in cargo substrates and mediates docking of the importin/substrate complex to the nuclear pore complex (NPC) through binding to nucleoporin. The complex is translocated through the pore by a Ran-dependent mechanism. At the nucleoplasmic side, Ran binds to importin, causing dissociation of the complex and re-export of importin to the cytoplasm, where GTP hydrolysis releases Ran. The directionality of nuclear import is conferred by asymmetric distribution of Ran forms between cytoplasm and nucleus. IPO11 mediates nuclear import of UBE2E3 and RPL12.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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