Thermo Fisher Scientific CD69 Monoclonal Antibody (H1.2F3), PE-Cyanine5, eBioscience

Applications and Tested Dilution

• Application: Flow Cytometry (Flow)
• Tested Dilution: 0.5 µg/test
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Product Specifications

Additional Formats Available

• Unconjugated
• Alexa Fluor 700
• APC
• APC-eFluor 780
• Biotin
• Brilliant UV 496 / 661 / 737
• Brilliant Violet 421 / 786
• eFluor 450
• FITC
• Functional Grade
• PE
• PE-Cyanine7
• PE-eFluor 610
• PerCP-Cyanine5.5
• Super Bright 436 / 600 / 645
• Request custom conjugation

Product Specific Information

The H1.2F3 monoclonal antibody reacts with mouse CD69 (very early activation antigen, VEA), a ~35 kDa disulfide-linked dimer expressed on activated lymphocytes. CD69 expression increases rapidly upon activation of T and B cells, indicating its role in lymphocyte activation and development.

• Applications Reported: Flow cytometric analysis
• Applications Tested: Tested on resting and activated mouse splenocytes at ≤0.5 µg/test (100 µL final volume). Optimal titration recommended.
• Light Sensitivity: Protect from light; tandem dye is photo-sensitive.
• Fixation: Samples can be stored up to 3 days at 4°C in the dark using IC Fixation Buffer (Product #00-8222-49) or 1-step Fix/Lyse Solution (Product #00-5333-54).
• Excitation: 488–561 nm
• Emission: 667 nm
• Compatible Lasers: Blue, Green, Yellow-Green
• Filtration: 0.2 µm post-manufacturing filtered

Target Information

CD69 (AIM, Active Inducer Molecule) is a gp28/34 disulfide-bonded homodimer (~60 kDa, non-reducing). It is present on activated platelets and involved in lymphocyte signal transduction. Expression is induced upon T lymphocyte activation and may play a role in cell proliferation and NK cell signaling.
Alternative splicing results in multiple transcript variants.
Diseases associated with CD69 dysfunction include coccidioidomycosis and asthma.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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