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Thermo Fisher Scientific CD69 Monoclonal Antibody (H1.2F3), PE-Cyanine5, eBioscience

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Flow cytometry용 마우스 CD69 단클론 항체(H1.2F3, PE-Cyanine5)로, 활성화된 T/B 세포의 표면 CD69 발현 분석에 적합합니다. 0.5 µg/test로 사용 가능하며, 4°C 암소 보관이 필요합니다. 연구용으로만 사용됩니다.

카탈로그번호: 15-0691-82
브랜드: Thermo Fisher Scientific
카테고리: Monoclonal Antibodies
판매단위: pk

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Thermo Fisher Scientific 15-0691-82 CD69 Monoclonal Antibody (H1.2F3), PE-Cyanine5, eBioscience 100 ug pk판매 단위 pk ·

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501,500원VAT 포함 551,650원

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Thermo Fisher Scientific · Thermo Fisher Scientific CD69 Monoclonal Antibody (H1.2F3), PE-Cyanine5, eBioscience

Applications and Tested Dilution

Application: Flow Cytometry (Flow)
Tested Dilution: 0.5 µg/test
View 60 publications

Product Specifications

항목	내용
Species Reactivity	Mouse
Published Species	Human, Mouse
Host / Isotype	Armenian hamster / IgG
Recommended Isotype Control	Armenian Hamster IgG Isotype Control (eBio299Arm), PE-Cyanine5, eBioscience™
Class	Monoclonal
Type	Antibody
Clone	H1.2F3
Conjugate	PE-Cyanine5
Excitation / Emission Max	568 / 666 nm
Form	Liquid
Concentration	0.2 mg/mL
Purification	Affinity chromatography
Storage Buffer	PBS, pH 7.2
Contains	0.09% sodium azide
Storage Conditions	4°C, store in dark, DO NOT FREEZE
Shipping Conditions	Ambient (domestic); Wet ice (international)
RRID	AB_468772

Additional Formats Available

Unconjugated
Alexa Fluor 700
APC
APC-eFluor 780
Biotin
Brilliant UV 496 / 661 / 737
Brilliant Violet 421 / 786
eFluor 450
FITC
Functional Grade
PE
PE-Cyanine7
PE-eFluor 610
PerCP-Cyanine5.5
Super Bright 436 / 600 / 645
Request custom conjugation

Product Specific Information

The H1.2F3 monoclonal antibody reacts with mouse CD69 (very early activation antigen, VEA), a ~35 kDa disulfide-linked dimer expressed on activated lymphocytes. CD69 expression increases rapidly upon activation of T and B cells, indicating its role in lymphocyte activation and development.

Applications Reported: Flow cytometric analysis
Applications Tested: Tested on resting and activated mouse splenocytes at ≤0.5 µg/test (100 µL final volume). Optimal titration recommended.
Light Sensitivity: Protect from light; tandem dye is photo-sensitive.
Fixation: Samples can be stored up to 3 days at 4°C in the dark using IC Fixation Buffer (Product #00-8222-49) or 1-step Fix/Lyse Solution (Product #00-5333-54).
Excitation: 488–561 nm
Emission: 667 nm
Compatible Lasers: Blue, Green, Yellow-Green
Filtration: 0.2 µm post-manufacturing filtered

Target Information

CD69 (AIM, Active Inducer Molecule) is a gp28/34 disulfide-bonded homodimer (~60 kDa, non-reducing). It is present on activated platelets and involved in lymphocyte signal transduction. Expression is induced upon T lymphocyte activation and may play a role in cell proliferation and NK cell signaling.
Alternative splicing results in multiple transcript variants.
Diseases associated with CD69 dysfunction include coccidioidomycosis and asthma.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.