Thermo Fisher Scientific Phospho-Syk (Tyr323) Polyclonal Antibody
인산화된 Syk(Tyr323)을 특이적으로 인식하는 Rabbit Polyclonal 항체로, WB, IHC, ICC/IF에 사용 가능. Human, Mouse, Rat 반응성. 고순도 Affinity chromatography 정제. PBS/glycerol buffer로 안정적 보관 가능.
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Thermo Fisher Scientific Phospho-Syk (Tyr323) Polyclonal Antibody
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:100 |
| Immunocytochemistry (ICC/IF) | 1:100–1:200 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Peptide sequence around phosphorylation site of tyrosine 323 (N-P-Y(p)-E-P) derived from Human Syk |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.4, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2554354 |
Product Specific Information
- Suggested positive control for Western blot: JK cells
- Suggested positive control for IHC: Human breast carcinoma
- Suggested positive control for ICC/IF: HeLa cells
Target Information
Spleen tyrosine kinase (SYK), along with ZAP70, are cytoplasmic tyrosine kinases considered potential drug targets for allergic and autoimmune diseases. SYK may also play a role in cancer. Upon B cell antigen receptor (BCR) or high-affinity IgE receptor (FcεRI) activation, Syk translocates to the plasma membrane and phosphorylates downstream adaptor proteins, initiating signaling pathways such as calcium mobilization, cytoskeleton remodeling, and gene transcription. Syk binds receptor assemblies via SH2 domain interactions with ITAM motifs phosphorylated by Src-family kinases, which also activate Syk through phosphorylation of its activation loop.
Usage Note
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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