Thermo Fisher Scientific ATF7 Polyclonal Antibody
Rabbit polyclonal antibody specific to Human ATF7 protein. Suitable for IHC (Paraffin) applications. Purified by antigen affinity chromatography. Supplied as liquid in PBS with glycerol and sodium azide. For research use only.
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Applications
- Immunohistochemistry (Paraffin) (IHC (P)): Tested dilution 1:200–1:500
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant protein corresponding to Human ATF7. Recombinant protein control fragment (Product #RP-100746) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 40% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2638325 |
Product Specific Information
Immunogen sequence:
LPGPPVQMPS VISLARPVSM VPNIPGIPGP PVNSSGSISP SGHPIPSEAK MRLKATLTHQ VSSINGGCGM VVGTASTMVT ARPEQSQILI QHPDAPSPAQ PQVSPAQPTP STGGRRRRTV DEDPDERRQR FLE
Highest antigen sequence identity to the following orthologs:
- Mouse: 98%
- Rat: 98%
Target Information
ATF7 plays important functions in early cell signaling. It binds the cAMP response element (CRE) (consensus: 5'-GTGACGT[AG][AG]-3'), a sequence found in many viral and cellular promoters. It acts as an activator of the NF-ELAM1/delta-A site of the E-selectin promoter. Although it lacks intrinsic transcriptional activity, ATF7 activates transcription when forming heterodimers with JUN or FOS. It can also bind TRE promoter sequences in complex with JUN family members.
Isoform 4 (ATF-A0) functions as a dominant repressor of the E-selectin/NF-ELAM1/delta-A promoter, while Isoform 5 (ATF-4) negatively regulates ATF2 and ATF7 transcriptional activities, possibly through cytoplasmic sequestration of the Thr-53 phosphorylating kinase, preventing activation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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