Thermo Fisher Scientific FOXP3 Monoclonal Antibody (FJK-16s), eFluor 615, eBioscience

Applications

Tested Dilution

Publications

Western Blot (WB)

-

View 12 publications 12 publications

Immunohistochemistry (IHC)

-

View 72 publications 72 publications

Immunohistochemistry (Paraffin) (IHC (P))

-

View 2 publications 2 publications

Immunohistochemistry (Frozen) (IHC (F))

10 µg/mL

-

Immunocytochemistry (ICC/IF)

Assay-Dependent

View 8 publications 8 publications

Flow Cytometry (Flow)

-

View 2 publications 2 publications

Immunoprecipitation (IP)

-

View 2 publications 2 publications

ChIP assay (ChIP)

-

View 2 publications 2 publications

In Situ Hybridization (ISH)

-

View 1 publication 1 publication

Product Specifications

Species Reactivity

Bovine, Dog, Cat, Mouse, Pig, Rat

Published species

Human, Mouse

Host/Isotype

Rat / IgG2a, kappa

Recommended Isotype Control

Rat IgG2a kappa Isotype Control (eBR2a), eFluor™ 615, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

FJK-16s

Conjugate

eFluor™ 615 eFluor™ 615 eFluor™ 615

View additional formats

• Unconjugated
• Alexa Fluor 488
• Alexa Fluor 532
• Alexa Fluor 561
• Alexa Fluor 660
• Alexa Fluor 700
• APC
• Biotin
• Brilliant Violet 421
• Brilliant Violet 605
• Brilliant Violet 711
• Brilliant Violet 786
• eFluor 450
• eFluor 506
• eFluor 570
• eFluor 660
• FITC
• PE
• PE-Cyanine5
• PE-Cyanine5.5
• PE-Cyanine7
• PE-eFluor 610
• PerCP-Cyanine5.5
• PerCP-eFluor 710
• Request custom conjugation

Excitation/Emission Max

595/614 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10804396

Product Specific Information

Description: The FJK-16s antibody reacts with mouse, rat, dog, porcine, bovine and cat Foxp3 also known as FORKHEAD BOX P3, SCURFIN, and JM2; cross reactivity of this antibody to other proteins has not been determined. Foxp3, a 49-55 kDa protein, is a member of the forkhead/winged-helix family of transcriptional regulators, and was identified as the gene defective in scurfy (sf) mice. Constitutive high expression of foxP3 mRNA has been shown in CD4+CD25+ regulatory T cells (Treg cells), and ectopic expression of foxp3 in CD4+CD25- cells imparts a Treg phenotype in these cells.

Immunoblotting with FJK-16s antibody has mapped the epitope to amino acids 75-125 of the mouse Foxp3 protein. In the human, this region has been shown to be alternatively spliced at the mRNA level. Both the alternatively-spliced and non-spliced isoforms are present in the CD4+CD25+ subset of lymphocytes. Preliminary RT-PCR experiments have not revealed this alternatively-spliced isoform in mouse splenocytes, suggesting different gene regulation in the mouse and human.

Applications Reported: This FJK-16s antibody has been reported for use in immunohistochemical staining of frozen tissue (IHC-F), and immunocytochemistry (ICC).

Applications Tested: This FJK-16s antibody has been tested by immunohistochemistry on frozen mouse spleen (IHC-F) at less than or equal to 10 µg/mL. This product has not been validated for flow cytometric analysis.

Filter Recommendation: When using this eFluor® 615 antibody conjugate, we recommend a filter that will capture the 615 emission wavelength (for example, Excitation 560/55, 585LP, Emission 645/75). A standard Alexa Fluor® 594 filter is acceptable.

Excitation: 595 nm; Emission: 615 nm.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

FOXP3 (Forkhead box protein 3) is a member of the forkhead/winged-helix family of transcriptional regulators, highly conserved across mammals, and essential for normal immune homeostasis. FOXP3 is 381 amino acids long, stably and constitutively expressed at a high level in CD25 + CD4 positive regulatory T cells, a low level in CD4-positive/CD25-negative cells, and is absent in CD4-negative/CD8-positive T cells. FOXP3 may be a master regulatory gene, and a more specific marker of regulatory T cells. Defects in the gene encoding FOXP3 protein cause the scurfy phenotype in mice. In humans FOXP3 defects play a role in IPEX syndrome (immune dysfunction, polyendocrinopathy, enteropathy, X-linked syndrome), also known as X-linked autoimmunity-allergic dysregulation (XLAAD) syndrome. Transcript variants of FOXP3 encoding different isoforms have been identified. In human breast and colon cancer cells, expression of FOXP3 is regulated by p53 in response to the DNA damage.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.