Thermo Fisher Scientific FOXP3 Monoclonal Antibody (FJK-16s), eFluor 615, eBioscience
FOXP3 단백질을 인식하는 FJK-16s 단클론 항체로, eFluor 615 형광으로 표지되어 있습니다. 마우스 및 다양한 종의 FOXP3 검출에 적합하며, IHC-F 및 ICC에 사용 가능합니다. 고순도 정제 및 안정적 형광 특성을 제공합니다.
- 카탈로그번호
- 42-5773-82
- 판매단위
- pk
카탈로그
1개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific FOXP3 Monoclonal Antibody (FJK-16s), eFluor 615, eBioscience
Thermo Fisher Scientific FOXP3 Monoclonal Antibody (FJK-16s), eFluor 615, eBioscience
Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | - | 12 |
| Immunohistochemistry (IHC) | - | 72 |
| Immunohistochemistry (Paraffin) (IHC (P)) | - | 2 |
| Immunohistochemistry (Frozen) (IHC (F)) | 10 µg/mL | - |
| Immunocytochemistry (ICC/IF) | Assay-Dependent | 8 |
| Flow Cytometry (Flow) | - | 2 |
| Immunoprecipitation (IP) | - | 2 |
| ChIP assay (ChIP) | - | 2 |
| In Situ Hybridization (ISH) | - | 1 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Bovine, Dog, Cat, Mouse, Pig, Rat |
| Published Species | Human, Mouse |
| Host / Isotype | Rat / IgG2a, kappa |
| Recommended Isotype Control | Rat IgG2a kappa Isotype Control (eBR2a), eFluor™ 615, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | FJK-16s |
| Conjugate | eFluor™ 615 |
| Excitation / Emission Max | 595 / 614 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_10804396 |
Available Conjugate Formats
- Unconjugated
- Alexa Fluor 488, 532, 561, 660, 700
- APC, Biotin
- Brilliant Violet 421, 605, 711, 786
- eFluor 450, 506, 570, 660
- FITC, PE, PE-Cyanine5, PE-Cyanine5.5, PE-Cyanine7
- PE-eFluor 610, PerCP-Cyanine5.5, PerCP-eFluor 710
- Custom conjugation available upon request
Product Specific Information
The FJK-16s antibody reacts with mouse, rat, dog, porcine, bovine, and cat Foxp3 proteins (FORKHEAD BOX P3, SCURFIN, JM2). Foxp3, a 49–55 kDa transcriptional regulator, is defective in scurfy mice. It is highly expressed in CD4⁺CD25⁺ regulatory T cells (Treg) and induces a Treg phenotype when expressed in CD4⁺CD25⁻ cells.
Epitope mapping shows reactivity to amino acids 75–125 of mouse Foxp3. Human Foxp3 shows alternative splicing in this region. Both isoforms are present in CD4⁺CD25⁺ lymphocytes. Mouse splenocytes lack this spliced isoform, suggesting species-specific regulation.
Applications Reported: Immunohistochemistry (frozen tissue, IHC-F) and Immunocytochemistry (ICC).
Applications Tested: Tested on frozen mouse spleen at ≤10 µg/mL. Not validated for flow cytometry.
Filter Recommendation: Use filter capturing 615 nm emission (e.g., Excitation 560/55, 585LP, Emission 645/75). Alexa Fluor® 594 filter acceptable.
Excitation/Emission: 595 nm / 615 nm
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
FOXP3 (Forkhead box protein 3) is a transcriptional regulator essential for immune homeostasis. It is highly expressed in CD25⁺CD4⁺ regulatory T cells, minimally in CD4⁺CD25⁻ cells, and absent in CD4⁻CD8⁺ T cells. FOXP3 mutations cause the scurfy phenotype in mice and IPEX syndrome in humans. Transcript variants encoding different isoforms exist, and FOXP3 expression in human cancer cells is regulated by p53 in response to DNA damage.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
제품 이미지
Thermo Fisher Scientific 상품 둘러보기
전체보기
문의
0개 · 배송·재고 문의는 실시간 상담이 빠릅니다아직 등록된 문의가 없어요.