Thermo Fisher Scientific CD3e Polyclonal Antibody

Applications

Immunohistochemistry (Paraffin) (IHC (P))

• Tested Dilution: 1:10–1:20

Product Specifications

Product Specific Information

PA5-16655 targets CD3E in IHC (P) applications and shows reactivity with human samples.
The immunogen is a synthetic 13-mer peptide corresponding to amino acids 156–168 of the epsilon-chain of human CD3 protein.

Target Information

The CD3 subunit complex is crucial for transducing antigen-recognition signals into the cytoplasm of T cells and for regulating TCR complex expression.
T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of CD3γ, CD3δ, CD3ε, and CD3ζ subunits. These are structurally related members of the immunoglobulin superfamily encoded by closely linked genes on chromosome 11.

CD3 components have long cytoplasmic tails that associate with signal transduction molecules, mediated by a double tyrosine-based motif.
CD3 may play roles in TCR-induced growth arrest, cell survival, and proliferation.

The CD3 antigen is present on:

• 68–82% of normal peripheral blood lymphocytes
• 65–85% of thymocytes
• Purkinje cells in the cerebellum

It is not expressed on B or NK cells.
Decreased percentages of T lymphocytes may be observed in some autoimmune diseases.
Defects in the CD3 gene are associated with CD3 immunodeficiency.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.