Gibco™

MAX Efficiency™ DH10Bac Competent Cells

MAX Efficiency DH10Bac Competent Cells are used for production of recombinant bacmids used in the Bac-to-Bac Baculovirus Expression System. The자세히 알아보기

MAX Efficiency DH10Bac Competent Cells are used for production of recombinant bacmids used in the Bac-to-Bac Baculovirus Expression System. The DH10Bac E. coli strain contains a baculovirus shuttle vector (bacmid) that can recombine with a donor plasmid, pFastBac, to create an expression bacmid containing a cloned gene of interest. Features of MAX Efficiency DH10Bac Competent Cells include:

• Consistent yields of >1 × 108 transformants/μg
• Tolerance of small amounts of undiluted ligation reactions
• Support of efficient blue/white colony screening (Φ80_lac_ZΔM15 marker)

Using MAX Efficiency DH10Bac competent cells
MAX Efficiency DH10Bac Competent Cells can serve as a host for a recombinant pFastBac vector containing a cloned gene of interest. DH10Bac cells harbor a baculovirus shuttle vector (bMON14272) and a helper plasmid (pMON7142), and are capable of supporting site-specific recombination between pFastBac and bMON14272 to generate high molecular weight bacmids that can then be amplified, purified, and used for insect cell transfection and subsequent gene expression. Kanamycin resistance for bacmid selection and maintenance is conferred by bMON14272, and tetracycline resistance by pMON7124.

Genotype:F-mcr_A Δ(_mrr_-hsdRMS-_mcr_BC) Φ80_lac_ZΔM15 Δ_lac_X74 _rec_A1 _end_A1 _ara_D139 Δ(_ara, leu)7697 _gal_U _gal_K λ-_rps_L _nup_G/pMON14272/pMON7124

사양

제품 유형Competent Cells

에프에피솜 포함Lacks F` Episome

효율> 1x10^8

세포 유형Chemically Competent

플라스미드 품질 개선No

메틸화 DNA 클로닝No

형질전환 효율 수준Medium Efficiency (10^8-10^9 cfu⁄μg)

불안정 DNA 클로닝Not Suitable for cloning unstable DNA

박테리아 또는 효모 균주DH10Bac

블루/화이트 스크리닝Yes

고처리량 호환성Not High-throughput Compatible (Manual)

비메틸화 DNA 준비Not Suitable for preparing unmethylated DNA

ccdB 벡터 증식Not for ccdB vector propagation

재조합 감소No

배송 조건Dry Ice

T1 Phage - 저항성(tonA)No

종E. coli

형식Tube

제품라인DH10Bac, MAX Efficiency™

수량5 x 100 μL

Unit SizeEach