ELK Biotechnology PDHK1 rabbit pAb
PDHK1 단백질을 인식하는 토끼 폴리클로날 항체로, WB, IHC, IF, ELISA에 적합합니다. 인간 유래 펩타이드로 면역화되었으며, 세포막 및 세포질에서 발현되는 PDK1 단백질 검출에 유용합니다. -20°C에서 1년 보관 가능합니다.
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PDHK1 rabbit pAb
제품 개요
ELK Biotechnology의 PDHK1 rabbit pAb는 인간 PDK1 단백질을 인식하는 토끼 유래 폴리클로날 항체입니다. Western blot, 면역조직화학, 면역형광, ELISA 등 다양한 응용에 적합합니다.
제품 정보
| 항목 | 내용 |
|---|---|
| Product name | PDHK1 rabbit pAb |
| Alternative Names | PDPK1; PDK1; 3-phosphoinositide-dependent protein kinase 1; hPDK1 |
| Applications | WB; IHC; IF; ELISA |
| Recommended Dilutions | WB: 1/500–1/2000 IHC: 1/100–1/300 ELISA: 1/5000 Not yet tested in other applications |
| Immunogen | Synthesized peptide derived from human PDK1 (AA range: 1–50) |
| Host | Rabbit |
| Storage | -20°C, 1 year |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1 mg/ml |
| Observed Band | 60 kDa |
| Gene ID (Human) | 5170 |
| Human Swiss-Prot No. | O15530 |
| Species Reactivity | Human, Mouse, Rat, Monkey |
세포 내 위치 (Cellular Localization)
Cytoplasm, nucleus, cell membrane (peripheral membrane protein), and cell junctions (focal adhesion).
Tyrosine phosphorylation occurs mainly at the cell membrane. Translocation to the membrane follows insulin stimulation via GRB14 and INSR binding. SRC and HSP90 promote membrane localization.
Nuclear localization depends on association with PTPN6 and phosphorylation at Ser-396.
In neuronal cells, restricted to nucleus; in non-neuronal cells, found in cytoplasm.
Ser-241 phosphorylated form localizes perinuclearly in neuronal cells and both nucleus and cytoplasm in non-neuronal cells.
IGF1 transiently increases phosphorylation at Ser-241 of neuronal PDPK1.
Background
- Catalytic activity: ATP + a protein → ADP + a phosphoprotein
- Function: Phosphorylates and activates PKB/AKT, PKA, PKC-zeta, RPS6KA1, and RPS6KB1. Plays a general role in signaling and development.
- PTM: Phosphorylated on tyrosine and serine/threonine residues. Ser-241 phosphorylation is required for full activity and can occur via autophosphorylation.
- Similarity: Belongs to the protein kinase superfamily, AGC Ser/Thr protein kinase family, PDK1 subfamily. Contains one PH domain and one protein kinase domain.
- Subcellular location: Membrane-associated after stimulation; tyrosine phosphorylation mainly at plasma membrane.
- Subunit interaction: Interacts with TUSC4.
- Tissue specificity: Expressed ubiquitously.
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