ELK Biotechnology PDHK1 (phospho Tyr9) rabbit pAb
PDHK1 (phospho Tyr9) rabbit polyclonal antibody는 인간, 생쥐, 랫트 시료에 반응하며 IHC, IF, ELISA에 사용 가능. Tyr9 인산화 부위에 특이적이며 세포막 및 핵 위치 연구에 적합. 장기 보관 가능(-20°C, 1년).
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PDHK1 (phospho Tyr9) Rabbit pAb
제품 개요
ELK Biotechnology에서 제공하는 PDHK1 (phospho Tyr9) rabbit polyclonal antibody는 인간 PDK1의 Tyr9 인산화 부위를 인식하는 항체로, 다양한 응용 분야에서 사용 가능합니다.
제품 정보
| 항목 | 내용 |
|---|---|
| Product name | PDHK1 (phospho Tyr9) rabbit pAb |
| Alternative Names | PDPK1; PDK1; 3-phosphoinositide-dependent protein kinase 1; hPDK1 |
| Applications | IHC; IF; ELISA |
| Recommended Dilutions | Immunohistochemistry: 1/100 - 1/300 ELISA: 1/40000 Not yet tested in other applications |
| Immunogen | Synthesized peptide derived from human PDK1 around the phosphorylation site of Tyr9 (AA range: 1–50) |
| Species Reactivity | Human; Mouse; Rat |
| Host | Rabbit |
| Storage | -20°C / 1 year |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1 mg/ml |
| GeneID (Human) | 5170 |
| Human Swiss-Prot No | O15530 |
세포 내 위치 (Cellular Localization)
Cytoplasm, Nucleus, Cell membrane (Peripheral membrane protein), Cell junction, focal adhesion.
Tyrosine phosphorylation occurs only at the cell membrane. Translocates to the membrane following insulin stimulation via GRB14 and INSR binding. SRC and HSP90 promote membrane localization. Nuclear localization depends on association with PTPN6 and phosphorylation at Ser-396. Restricted to the nucleus in neuronal cells; cytoplasmic in non-neuronal cells. Ser-241 phosphorylated form is perinuclear in neuronal cells, nuclear and cytoplasmic in non-neuronal cells. IGF1 transiently increases phosphorylation at Ser-241 of neuronal PDPK1.
Background
- Catalytic activity: ATP + a protein → ADP + a phosphoprotein
- Function: Phosphorylates and activates PKB/AKT, PKA, PKC-zeta, RPS6KA1, RPS6KB1; plays a general role in signaling and development.
- PTM: Phosphorylated on tyrosine and serine/threonine; Ser-241 phosphorylation required for full activity; can autophosphorylate Ser-241.
- Similarity: Belongs to the protein kinase superfamily, AGC Ser/Thr protein kinase family, PDK1 subfamily. Contains 1 PH domain and 1 protein kinase domain.
- Subcellular location: Membrane-associated after stimulation; tyrosine phosphorylation occurs only at plasma membrane.
- Subunit interaction: Interacts with TUSC4.
- Tissue specificity: Ubiquitously expressed.
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