Thermo Fisher Scientific RFC1 Polyclonal Antibody
RFC1 단백질을 인식하는 Rabbit Polyclonal Antibody로, WB, IHC, ICC, ELISA 등 다양한 응용에 적합. Human, Mouse, Rat 시료 반응성. Protein A 정제, 1 mg/mL 액상 형태로 제공. DNA 복제 및 수선 연구용으로 적합.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC-P) | 1:200 |
| Immunohistochemistry (Frozen) (IHC-F) | 1:100–1:500 |
| Immunocytochemistry (ICC/IF) | 1:100–1:500 |
| ELISA | 1:500–1:1,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | KLH-conjugated synthetic peptide derived from human RFC1 (amino acids 401–500) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | 0.01M TBS, pH 7.4, with 50% glycerol, 1% BSA |
| Contains | 0.02% ProClin 300 |
| Storage Conditions | −20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
Replication factor C (RFC) is an essential DNA polymerase accessory protein required for multiple DNA metabolic processes including replication, repair, and telomere maintenance. RFC forms a heteropentameric complex that recognizes primer-template DNA, binds the primer terminus, and loads proliferating cell nuclear antigen (PCNA) onto DNA in an ATP-dependent manner. All five RFC subunits share conserved RFC box sequences with nucleotide-binding motifs. Four subunits (RFC1–RFC4) contain ATP-binding motifs. Smaller RFC proteins (RFC2–RFC5) interact with Rad24, while RFC1 does not. RFC2, the third-largest subunit, binds ATP and plays a role in DNA replication and checkpoint control.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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