Thermo Fisher Scientific AGO3 (Argonaute-3)/eIF2C3 Monoclonal Antibody (PCRP-AGO3-1C5)
AGO3 단백질을 표적으로 하는 인간 반응성 단클론 항체로 RNA 간섭 연구에 적합. ICC, Flow, ELISA, IP 등 다양한 응용 가능. 단백질 A/G로 정제된 액상 형태, 보존제 없음. -20°C에서 보관 권장.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Immunocytochemistry (ICC/IF) | 1–2 µg/mL |
| Flow Cytometry (Flow) | 1–2 µg/10⁶ cells |
| ELISA | Assay-dependent |
| Immunoprecipitation (IP) | 1–2 µg/100–500 µg total protein |
| Peptide Array | Assay-dependent |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | PCRP-AGO3-1C5 |
| Immunogen | Recombinant full-length human AGO3 protein (Argonaute 3, UniProt ID: Q9H9G7-1, amino acids 1–860) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4 |
| Contains | No preservative |
| Storage Conditions | -20°C, avoid freeze/thaw cycles |
| Shipping Conditions | Wet ice |
Target Information
AGO3 (Argonaute-3) is a protein required for RNA-mediated gene silencing (RNA interference). It binds short RNAs such as microRNAs (miRNAs) and represses translation of complementary mRNAs. AGO3 is proposed to stabilize small RNA derivatives (riRNA) derived from RNA polymerase III-transcribed Alu repeats containing a DR2 retinoic acid response element (RARE) in stem cells and to facilitate riRNA-dependent degradation of certain RNA polymerase II-transcribed mRNAs by recruiting an mRNA decapping complex involving EDC4.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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