Thermo Fisher Scientific AGO3 (Argonaute-3)/eIF2C3 Monoclonal Antibody (PCRP-AGO3-1C5)

Applications

Tested Dilution

Publications

Immunocytochemistry (ICC/IF)

1-2 µg/mL

-

Flow Cytometry (Flow)

1-2 µg/10^6 cells

-

ELISA (ELISA)

Assay-dependent

-

Immunoprecipitation (IP)

1-2 µg/100-500 µg total protein

-

Peptide Array (Array)

Assay-dependent

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

PCRP-AGO3-1C5

Immunogen

Recombinant full-length human AGO3 protein if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:Argonaute 3,uniProtId:Q9H9G7-1,ncbiNodeId:9606,antigenRange:1-860,antigenLength:860,antigenImageFileName:192669-MSM1-P1ABX_Argonaute_3_Q9H9G7-1_House_mouse.svg,antigenImageFileNamePDP:192669-MSM1-P1ABX_Argonaute_3_Q9H9G7-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A/G

Storage buffer

PBS, pH 7.4

Contains

no preservative

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

Target Information

AGO3 (protein argonaute-3) is required to RNA-mediated gene silencing (RNAi). It binds to short RNAs such as microRNAs (miRNAs) and represses the translation of mRNAs which are complementary to them. AGO3 is proposed to be involved in stabilization of small RNA derivates (riRNA) derived from processed RNA polymerase III-transcribed Alu repeats containing a DR2 retinoic acid response element (RARE) in stem cells and in the subsequent riRNA-dependent degradation of a subset of RNA polymerase II-transcribed coding mRNAs by recruiting a mRNA decapping complex involving EDC4.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.