Thermo Fisher Scientific Ion AmpliSeq Mouse TCR Beta SR Assay, DNA

Ion AmpliSeq™ Mouse TCR Beta SR Assay, DNA

The Ion AmpliSeq Mouse TCR Beta SR Assay is a robust, targeted next-generation sequencing (NGS) assay designed to accurately identify and measure the clonal expansion of T lymphocytes by targeting the complementarity-determining region 3 (CDR3) of the T-cell receptor (TCR) gene locus from gDNA input.
The assay is suitable for basic and translational research to identify T-cell clones, as the nucleotide sequence of the CDR3 region is unique to each T cell and encodes the part of the TCR beta chain involved in antigen recognition.
For RNA samples, please see Cat. No. A45489.

주요 특징

• 높은 민감도 및 특이성: 희귀 클론 식별이 가능한 듀얼 바코드 인덱싱 적용
• 유연한 입력량: 100 ng–1 μg의 DNA 입력 가능
• 다양한 샘플 호환성: FFPE 조직, 혈액, 말초혈액 단핵세포 등 사용 가능
• 효율적 워크플로우: 약 48시간 내 결과 확인 가능
• 통합 분석 솔루션: 다중 샘플 분석이 가능한 Ion Reporter 소프트웨어 지원

응용 분야

• 면역학 및 면역항암 연구
• 백신 및 CAR-T 연구
• 마우스 모델에서의 T 세포 반응 분석

제품 구성 및 워크플로우

The Ion AmpliSeq TCR Beta-SR Mouse Kit, DNA, provides a single pool of multiplex PCR primers and library reagents to generate 90-bp amplicons.
These can be sequenced on all chip types supported by the Ion GeneStudio S5 sequencing systems, allowing flexible sample batching and throughput.
The entire workflow—from library preparation to sample analysis—can be completed within two days using the Ion Chef templating system and Ion GeneStudio S5 sequencing system.

Learn more about the assay ›

Note: Information about data analysis using Ion Reporter Software v5.12 can be found in the User Guide.

사양