Thermo Fisher Scientific IL-17A Monoclonal Antibody (eBio17B7), eFluor 450, eBioscience
Mouse와 Rat IL-17A에 특이적인 eBio17B7 단클론 항체로, eFluor 450 형광체가 결합되어 Flow Cytometry에 최적화됨. IL-17A 관련 염증 반응 연구에 활용 가능하며, Violet laser(405 nm)에서 445 nm 방출. Affinity chromatography로 정제된 고품질 항체.
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Thermo Fisher Scientific IL-17A Monoclonal Antibody (eBio17B7), eFluor 450, eBioscience
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (IHC) | - | View 5 publications |
| Immunohistochemistry (Paraffin) (IHC (P)) | - | View 1 publication |
| Flow Cytometry (Flow) | 0.25 µg/test | View 91 publications |
| ELISA | - | View 3 publications |
| Functional Assay | - | View 1 publication |
Product Specifications
| Specification | Detail |
|---|---|
| Species Reactivity | Mouse, Rat |
| Published Species | Mouse |
| Host / Isotype | Rat / IgG2a, kappa |
| Recommended Isotype Control | Rat IgG2a kappa Isotype Control (eBR2a), eFluor™ 450 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | eBio17B7 |
| Conjugate | eFluor™ 450 |
| Excitation / Emission Max | 405 / 445 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_11149677 |
Available Conjugate Formats
- Alexa Fluor 488
- APC
- Biotin
- Brilliant UV 395, 615, 661, 737, 805
- Brilliant Violet 421, 650, 711, 786
- eFluor 506, 660
- FITC
- PE, PE-Cyanine7, PE-eFluor 610
- PerCP-Cyanine5.5
- Custom conjugation available
Product Specific Information
The eBio17B7 antibody reacts with mouse and rat IL-17A without cross-reactivity to IL-17F. IL-17A is a CD4+ T cell-derived cytokine that promotes inflammatory responses and is elevated in autoimmune and inflammatory diseases such as rheumatoid arthritis, asthma, multiple sclerosis, psoriasis, and transplant rejection.
IL-17A enhances ICAM-1 expression and induces secretion of IL-6, IL-8, G-CSF, and PGE2 in epithelial and fibroblastic cells. It also supports proliferation of CD34+ hematopoietic progenitors and their maturation into neutrophils.
IL-23-dependent Th17 cells produce IL-17A, distinct from Th1 and Th2 differentiation pathways.
Applications Reported:
- Suitable for intracellular staining followed by flow cytometric analysis.
Applications Tested:
- Tested on Th17-polarized mouse splenocytes using Intracellular Fixation and Permeabilization Buffer Set (Product # 88-8824-00).
- Recommended usage ≤ 0.25 µg/test in 100 µL final volume.
- Optimize antibody titration for best performance.
- Compatible with Foxp3 buffer system (Product # 00-5523-00).
Fluorochrome Information:
- eFluor® 450 is an alternative to Pacific Blue®.
- Excitation: 405 nm; Emission: 445 nm; Laser: Violet.
- Post-manufacturing filtered at 0.2 µm.
Target Information
Interleukin-17A (IL-17A, CTLA-8) is a CD4+ T cell-derived cytokine involved in inflammatory responses. It forms a 32 kDa disulfide-linked homodimer and signals through IL-17R/CDw217. IL-17A regulates NF-kappa B and MAPK pathways, enhances IL-6 and COX-2 expression, and promotes nitric oxide production.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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