Thermo Fisher Scientific SREBP1 Polyclonal Antibody
SREBP1 단백질을 표적하는 Rabbit Polyclonal 항체로, Western blot, IHC, ICC 등 다양한 응용에 적합합니다. Human 및 Mouse 시료에 반응하며, 항원 친화 크로마토그래피로 정제되었습니다. 장기 보관 시 -20°C에서 보관 권장.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:500 |
| Immunocytochemistry (ICC/IF) | 1:50–1:100 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide within N-terminal human SREBP1 (aa 25–55) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | TBS, pH 7.4, with 50% glycerol, 0.2% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2932527 |
Target Information
Sterol regulatory element-binding proteins (SREBPs) are transcription factors belonging to the basic helix-loop-helix leucine zipper family of DNA-binding proteins. Three isoforms (SREBP-1a, SREBP-1c, SREBP-2) exist in mammals, differing in structure, regulation, and function.
SREBP-1a and SREBP-1c are products of alternative promoter usage from the SREBP-1 gene, while SREBP-2 originates from a separate gene.
SREBPs are synthesized as 120 kDa inactive precursors in the endoplasmic reticulum membrane. Upon activation, they are translocated to the Golgi, where proteolytic cleavage produces a 60–78 kDa transcriptionally active fragment.
In liver and adipose tissue, SREBPs regulate lipid and cholesterol metabolism by inducing transcription of related genes. SREBP-1 primarily regulates triglyceride synthesis, while SREBP-2 is more closely associated with cholesterol synthesis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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