Thermo Fisher Scientific GCLC Monoclonal Antibody (3H1)

Applications

Tested Dilution

Publications

Western Blot (WB)

1-5 µg/mL

-

Immunocytochemistry (ICC/IF)

10 µg/mL

-

ELISA (ELISA)

0.03 ng/mL

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

3H1

Immunogen

GCLC (NP_001489, 528 a.a. approximately 637 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:GCLC,uniProtId:P48506-1,ncbiNodeId:9606,antigenRange:528-637,antigenLength:637,antigenImageFileName:H00002729-M01_GCLC_P48506-1_House_mouse.svg,antigenImageFileNamePDP:H00002729-M01_GCLC_P48506-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

See Label

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.4

Contains

no preservative

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

Sequence of this protein is as follows: EGVFPGLIPI LNSYLENMEV DVDTRCSILN YLKLIKKRAS GELMTVARWM REFIANHPDY KQDSVITDEM NYSLILKCNQ IANELCECPE LLGSAFRKVK YSGSKTDSSN

Target Information

Glutamate-cysteine ligase, also known as gamma-glutamylcysteine synthetase is the first rate-limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. This locus encodes the catalytic subunit, while the regulatory subunit is derived from a different gene located on chromosome 1p22-p21. Mutations at this locus have been associated with hemolytic anemia due to deficiency of gamma-glutamylcysteine synthetase and susceptibility to myocardial infarction.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.