Thermo Fisher Scientific PNUTS Polyclonal Antibody
Thermo Fisher Scientific의 PNUTS Polyclonal Antibody는 Human에 반응하며 Western blot, IHC, IP 등 다양한 응용에 적합합니다. Rabbit IgG 기반의 비결합 항체로, 항원 친화 크로마토그래피로 정제되었습니다. 4°C에서 보관하며 연구용으로 사용됩니다.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000–1:10,000 |
| Immunohistochemistry (IHC) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:500–1:2,000 |
| Immunoprecipitation (IP) | 1–10 µg/mg lysate |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Human |
| Host/Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Region between residues 350 and 400 of Human Phosphatase Nuclear Targeting Subunit (Protein Phosphatase 1, regulatory subunit 10) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | Tris citrate/phosphate, pH 7–8 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
Product Specific Information
- Recommended shelf life: 1 year from date of receipt
- For IHC, epitope retrieval with citrate buffer (pH 6.0) is recommended for FFPE tissue sections
- Predicted to react with Rat, Monkey, and Chimpanzee based on 100% sequence identity
Target Information
RNA polymerases transcribe nuclear genes for ribosomal RNA, representing ribosomal biogenesis. RNA polymerase I (Pol I) is located in the nucleolus and transcribes class I genes coding for large ribosomal RNA.
Different subunits of the Pol I transcription machinery are targets of various physiological stimuli, suggesting multiple signaling pathways are involved in Pol I transcription.
RPA40 and RPA16 are subunits of Pol I that associate early in RNA polymerase I assembly. RPA40 is essential for the function and integrity of the complex and is also a subunit of RNA polymerase III (Pol III).
RPA40, RPA16, and RPA135 encode three subunits of RNA polymerase I, respectively. RPA194 is the largest subunit of RNA Pol I and is not a component of Pol II or Pol III.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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