Thermo Fisher Scientific FIS1 Monoclonal Antibody (GT12112)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:3,000

-

Immunocytochemistry (ICC/IF)

1:100-1:1,000

-

Product Specifications

Species Reactivity

Human, Mouse

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

GT12112

Immunogen

Full length human FIS1 Recombinant protein. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:FIS1,uniProtId:Q9Y3D6-1,ncbiNodeId:9606,antigenRange:1-152,antigenLength:152,antigenImageFileName:MA5-27834_FIS1_Q9Y3D6-1_House_mouse.svg,antigenImageFileNamePDP:MA5-27834_FIS1_Q9Y3D6-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

3.55 mg/mL

Purification

Protein G

Storage buffer

PBS

Contains

no preservative

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2735184

Product Specific Information

Positive Control: HeLa, NCI-H929

Predicted Reactivity: Mouse (96%), Rat (87%), Rhesus Monkey (100%), Bovine (97%)

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

Fis1, a 17KDa integral membrane protein, is a novel member of mitochondrial fission machinery. It consists of a central leucine-zipper domain, a coiled-coil region, a tetratricopeptide repeat domain and a C-terminal transmembrane domain. It is a human orthologue of Fis1p (S.cerevisiae) and is localized in the outer mitochondrial membrane. Fis1 acts as a pro-apoptotic factor and plays a vital role in the regulation of mitochondrial fission. It interacts with DLP1 and Mdv1p and facilitates Dnm1p recruitment to the functional fission complex, thereby inducing apoptosis. Role of Fis1 has also been implicated in growth and division of peroxisomes. Loss of Fis1 inhibits apoptosis and results in elongation of mammalian mitochondria.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.