Thermo Fisher Scientific NSUN2 Polyclonal Antibody
Rabbit polyclonal antibody targeting human and mouse NSUN2 protein. Validated for Western blot at 1:1,000 dilution. Recognizes central region (aa 423–451) of NSUN2. Supplied as liquid, 0.45 mg/mL, in PBS with sodium azide. Suitable for research use only.
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Applications
- Western Blot (WB): 1:1,000 dilution
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | KLH-conjugated synthetic peptide corresponding to amino acids 423–451 of the central region of human NSUN2 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.45 mg/mL |
| Purification | Protein A, Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.4 |
| Contains | 0.09% sodium azide |
| Storage Conditions | Store at 4°C short term. For long-term storage, store at -20°C. Avoid freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2541800 |
Target Information
NSUN2 encodes a methyltransferase that catalyzes the intron-dependent formation of 5-methylcytosine at C34 of tRNA-leu(CAA). Maturation of cytoplasmic tRNAs includes splicing of introns located one nucleotide 3′ from the anticodon in all intron-containing tRNA genes. In tRNA-leu(CAA), the first position of the anticodon (C34) is converted to 5-methylcytosine, a modification essential for stabilizing anticodon–codon pairing and accurate mRNA translation. Mutations in NSUN2 are associated with autosomal recessive mental retardation (MRT5).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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