Thermo Fisher Scientific MKNK2 Monoclonal Antibody (4F11)

Applications

Tested Dilution

Publications

Western Blot (WB)

1-5 µg/mL

-

Immunocytochemistry (ICC/IF)

25 µg/mL

-

ELISA (ELISA)

1 ng/mL

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgG2a, kappa

Class

Monoclonal

Type

Antibody

Clone

4F11

Immunogen

MKNK2 (AAH18345, 1 a.a. approximately 158 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:MKNK2,uniProtId:Q9HBH9-1,ncbiNodeId:9606,antigenRange:1-465,antigenLength:465,antigenImageFileName:H00002872-M04_MKNK2_Q9HBH9-1_House_mouse.svg,antigenImageFileNamePDP:H00002872-M04_MKNK2_Q9HBH9-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

See Label

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.4

Contains

no preservative

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

Sequence of this protein is as follows: MAPEVVEAFS EEASIYDKRC DLWSLGVILY ILLSGYPPFV GRCGSDCGWD RGEACPACQN MLFESIQEGK YEFPDKDWAH ISCAAKDLIS KLLVRDAKQR LSAAQVLQHP WVQGCAPENT LPTPMVLQRW DSHFLLPPHP CRIHVRPGGL VRTVTVNE

Target Information

The Map kinase-interacting kinases (MKNK1 and MKNK2) are activated by Map kinases, regulate protein synthesis by phosphroylation eIF4E on Ser209, and may contribute to tumorigenesis. Overexpression of Mnk2 results in increased phosphorylation of endogenous eIF-4E, showing that it can act as an eIF-4E kinase in vivo. Mnk2 may play a role in the response to environmental stress and cytokines. Expression of active mutants of MNK1 and MNK2 in 293 cells diminishes cap-dependent translation relative to cap-independent translation in a transient reporter assay. Human Mnk2 is homologous to murine Mnk2 (approximately 94% identical) and human Mnk1 (71% identical). In vitro phosphorylation studies show that Mnk2 is a significantly better substrate than Mnk1 for extracellular signal-regulated kinase 2 (Erk2), p38MAPKalpha, and p38MAPKbeta.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.