
Thermo Fisher Scientific Goat anti-Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, APC
APC 형광단을 결합한 염소 유래 항-마우스 IgG(H+L) 2차 항체로, IHC·ICC·Flow Cytometry에 적합. 사람 IgG에 교차흡착 처리되어 비특이적 반응 최소화. 높은 광안정성과 633 nm 최적 여기 특성으로 정밀 형광 분석에 유용.
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Thermo Fisher Scientific Goat anti-Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, APC
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Immunohistochemistry (IHC) | 1–10 µg/mL |
| Immunocytochemistry (ICC/IF) | 2 µg/mL |
| Flow Cytometry (Flow) | 1–10 µg/mL |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Mouse |
| Host/Isotype | Goat / IgG |
| Class | Polyclonal |
| Type | Secondary Antibody |
| Immunogen | Gamma Immunoglobins Heavy and Light chains |
| Conjugate | APC |
| Excitation/Emission Max | 651 / 660 nm |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Purified |
| Storage Buffer | PBS, pH 7.5 |
| Contains | 5 mM Sodium Azide |
| Storage Conditions | 4°C, store in dark |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2536211 |
| Target | IgG |
| Cross Adsorption | Against human IgG and human serum |
| Antibody Form | Whole Antibody |
Product Specific Information
Allophycocyanin (APC)-conjugated goat anti-mouse IgG is prepared from affinity-purified antibodies that react with IgG heavy chains and all classes of light chains from mouse.
This phycobiliprotein is optimally excited at 633 nm but can also be excited at 594 nm, allowing use with Texas Red or Alexa Fluor 594 dyes without an additional excitation source.
APC is suitable for flow cytometry and imaging applications and offers superior photostability compared to Cy5 conjugates.
Target Information
Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins.
They are useful for detection, sorting, or purification of target proteins. Secondary antibodies provide versatility for multiple detection systems (HRP, AP, fluorescence) and enhance sensitivity through signal amplification.
Typically, host animals are immunized with pooled immunoglobulins from the target species and the antibodies are further purified or conjugated for high specificity.
For Research Use Only. Not for use in diagnostic procedures or resale without authorization.
Available Formats
A variety of conjugate formats are available, including:
- Agarose
- Alexa Fluor series (350–790)
- DyLight series (350–800)
- FITC, HRP, PE, Rhodamine, TRITC, Texas Red-X, and more
- Custom conjugation upon request
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