Invitrogen™

Click-iT™ Plus Alexa Fluor™ 647 Picolyl Azide Toolkit

Click-iT™ Plus Alexa Fluor™ picolyl azide toolkits contain everything you need to perform copper-catalyzed click reactions with compounds that are자세히 알아보기

Click-iT™ Plus Alexa Fluor™ picolyl azide toolkits contain everything you need to perform copper-catalyzed click reactions with compounds that are sensitive to copper, while retaining all of the benefits of the standard azide/alkyne click reaction. Picolyl azides react efficiently with chelated copper, so free copper in the click reaction can be minimized, protecting against undesired copper side reactions with proteins (e.g., GFP, RPE), nucleic acids (e.g., RNA, oligos), and even small molecules (e.g., phalloidin).

• Flexible—optimize your own Click–iT™ reaction for the detection or labeling of any alkyne-containing biomolecule in vitro or in cells and tissue samples
• Bright and sensitive—superior fluorescent Alexa Flour™ dyes combined with the efficiency of picolyl azide
• Convenient—contains all the buffers and reagents needed to create the optimal click reaction

Each Click-iT™ Plus Picolyl Azide Toolkit includes 100 μg of Alexa Fluor™ dye-picolyl azide and all of the reagents required to create a reaction cocktail with the optimal copper and chelate concentration for any click chemistry-based labeling or detection application. Guidelines for designing and optimizing the picolyl azide/alkyne click reaction of your choice are provided in the product manual. Additionally Click-iT™ Plus picolyl azides offer increased sensitivity and faster reaction times compared to standard azides using identical reaction conditions.

사양

설명Click-iT™ Plus Alexa Fluor™ 647 Picolyl Azide Toolkit, 5mg, Solid, 650nm Emission, 670nm Excitation, 1 Year Shelf Life, 2 to 8°C Storage Requirements, Less Hazardous Green Features, Used for Labeling Chemistry

용도(애플리케이션)Labeling chemistry, cell analysis, click chemistries, glycoprotein analysis with click chemistry, metabolic labeling and nascent protein synthesis with click chemistry, cell proliferation with click chemistry, nascent RNA detection and capture with click

키트 구성품1 x 100μg Alexa Fluor™ dye-picolyl azide, 1 x 4mL Click-iT™ reaction buffer, 1 x 1.5mL copper (II) sulfate, 1 x 500μL copper protectant, 1 Click-iT™ buffer additive

제품 유형Click-iT™ Plus Alexa Fluor™ 647 Picolyl Azide Toolkit

수량1 Kit

여기/방출650/670

제품라인Click-iT™

라벨 또는 염료Alexa Fluor 647

형식Solid

Unit SizeEach