Thermo Fisher Scientific eIF3l Polyclonal Antibody

Applications

Tested Dilution

Publications

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200

-

Immunocytochemistry (ICC/IF)

0.25-2 µg/mL

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein corresponding to Human eIF3l. Recombinant protein control fragment (Product #RP-91347). if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:eIF3l,uniProtId:Q9Y262-1,ncbiNodeId:9606,antigenRange:400-537,antigenLength:564,antigenImageFileName:PA5-51799_eIF3l_Q9Y262-1_Rabbit.svg,antigenImageFileNamePDP:PA5-51799_eIF3l_Q9Y262-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.2, with 40% glycerol

Contains

0.02% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2640969

Product Specific Information

Immunogen sequence: RMQKGDPQVY EELFSYSCPK FLSPVVPNYD NVHPNYHKEP FLQQLKVFSD EVQQQAQLST IRSFLKLYTT MPVAKLAGFL DLTEQEFRIQ LLVFKHKMKN LVWTSGISAL DGEFQSASEV DFYIDKDMIH IADTKVAR

Highest antigen sequence identity to the following orthologs: Mouse - 99%, Rat - 99%.

Target Information

Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis (PubMed:17581632, PubMed:25849773, PubMed:27462815). The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S pre-initiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation (PubMed:17581632). The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation, including cell cycling, differentiation and apoptosis, and uses different modes of RNA stem-loop binding to exert either translational activation or repression (PubMed:25849773). [UniProt]

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.