Thermo Fisher Scientific P4HA2 Polyclonal Antibody
Human P4HA2 단백질을 인식하는 Rabbit Polyclonal 항체로, WB, IHC(P), ICC/IF에 사용 가능. 항원 친화 크로마토그래피로 정제되었으며 PBS buffer에 보존. 콜라겐 합성 관련 연구용으로 적합.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.04–0.4 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 0.25–2 µg/mL |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant protein corresponding to Human P4HA2. Recombinant protein control fragment (Product #RP-89682). |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.09 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 40% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | Store at 4°C short term. For long-term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2645149 |
Product Specific Information
Immunogen sequence:
LDYLSYAVFQ LGDLHRALEL TRRLLSLDPS HERAGGNLRY FEQLLEEERE KTLTNQTEAE LATPEGIYER PVDYLPERDV YESLCRGEGV KLTPRRQKRL FCRYHHGNRA PQLLIAPFKE EDEWDSPHIV RYYDVMS
Sequence identity to orthologs:
- Mouse: 91%
- Rat: 90%
Target Information
This gene encodes a component of prolyl 4-hydroxylase, a key enzyme in collagen synthesis composed of two identical alpha subunits and two beta subunits. The encoded protein provides the major part of the catalytic site of the active enzyme. In collagen and related proteins, prolyl 4-hydroxylase catalyzes the formation of 4-hydroxyproline, which is essential for proper three-dimensional folding of newly synthesized procollagen chains. Alternatively spliced transcript variants encoding different isoforms have been described.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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