Thermo Fisher Scientific Goat anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, AP
Goat anti-Mouse IgG (H+L) AP-conjugated secondary antibody for WB, IHC, ELISA. Highly cross-adsorbed to minimize cross-reactivity. Affinity purified for high specificity and sensitivity. Suitable for research use only.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:20,000 |
| Immunohistochemistry (IHC) | 1:500–1:20,000 |
| ELISA | 1:500–1:20,000 |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Mouse |
| Host / Isotype | Goat / IgG |
| Class | Polyclonal |
| Type | Secondary Antibody |
| Immunogen | Gamma Immunoglobulins Heavy and Light chains |
| Conjugate | Alkaline Phosphatase (AP) |
| Form | Liquid |
| Concentration | 2.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | 30 mM triethanolamine, 1% BSA, 5 mM MgCl₂, 0.1 mM ZnCl₂ |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term; for long-term storage, store at -20°C avoiding freeze/thaw cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2534755 |
| Target | IgG |
| Cross Adsorption | Against bovine, goat, human, rabbit, and rat IgG |
| Antibody Form | Whole Antibody |
Additional Formats Available
- Alexa Fluor 350, 488, 546, 555, 568, 594, 633, 635, 647, 660, 680, 790
- Alexa Fluor Plus 405, 488, 555, 594, 647, 680, 800
- Biotin, DSB-X Biotin, FITC, HRP, TRITC
- Custom conjugation available upon request
Target Information
Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins. They are useful in detection, sorting, or purification of mouse IgG targets. Secondary antibodies provide versatility for various detection systems (HRP, AP, fluorescence) and enhance sensitivity through signal amplification, as multiple secondary antibodies can bind to a single primary antibody. These antibodies are typically generated by immunizing the host animal with pooled immunoglobulins from the target species and further purified or modified to achieve high specificity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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