Thermo Fisher Scientific DBT Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

0.04-0.4 µg/mL

-

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein corresponding to Human DBT. Recombinant protein control fragment (Product #RP-94247). if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:DBT,uniProtId:P11182-1,ncbiNodeId:9606,antigenRange:36-110,antigenLength:482,antigenImageFileName:PA5-55335_DBT_P11182-1_Rabbit.svg,antigenImageFileNamePDP:PA5-55335_DBT_P11182-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.2, with 40% glycerol

Contains

0.02% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2640397

Product Specific Information

Immunogen sequence: YVCFFGYPSF KYSHPHHFLK TTAALRGQVV QFKLSDIGEG IREVTVKEWY VKEGDTVSQF DSICEVQSDK ASVTI

Highest antigen sequence identity to the following orthologs: Mouse - 84%, Rat - 81%.

Target Information

The branched-chain alpha-keto acid dehydrogenase complex (BCKD) is an inner-mitochondrial enzyme complex involved in the breakdown of the branched-chain amino acids isoleucine, leucine, and valine. The BCKD complex is thought to be composed of a core of 24 transacylase (E2) subunits, and associated decarboxylase (E1), dehydrogenase (E3), and regulatory subunits. This gene encodes the transacylase (E2) subunit. Mutations in this gene result in maple syrup urine disease, type 2. Alternatively spliced transcript variants have been described, but their biological validity has not been determined.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.