Invitrogen™

MultiShot™ TOP10 Chemically Competent E. coli

MultiShot TOP10 Chemically Competent E. coli cells are derived from DH10B and are one of the most popular laboratory strains.자세히 알아보기

MultiShot TOP10 Chemically Competent E. coli cells are derived from DH10B and are one of the most popular laboratory strains. Packaged in five 96-well microtiter plates (15 μL aliquots) to simplify high-throughput workflows and provide transformation efficiencies of >1 x 108 cfu/μg control plasmid DNA, Multishot TOP10 is a versatile strain that can be used in multiple routine cloning applications.

Benefits of MultiShot TOP10 competent cells
• TOP10 strain is ideal for high-efficiency cloning and plasmid propagation
• Microtiter plate format design is convenient for automated high-throughput cloning
• _mcr_A mutation supports efficient transfection of methylated DNA
• _l__ac_ZΔM15 allele permits rapid blue/white screening of transfectants
• _end_A1 mutation reduce endonuclease activity and improve plasmid DNA yields
• _rec_A1 mutation helps reduce unwanted recombination

Easy to use for high-throughput transformations
MultiShot TOP10 Chemically Competent cells in 96-well plates are designed to facilitate high-throughput transformation. This format allows all steps of the transformation protocol, up to plating, to take place in the same well, saving time and reducing the chance of contamination. After the addition of DNA, MultiShot TOP10 Chemically Competent cells can be transformed by heat-shock at 42°C using a heat block or thermocycler in the same plate.

Using our TA Cloning and TOPO Cloning protocols, including controls, yields of 100–400 colonies per agar plate can be expected.

Genotype
F-mcr_A Δ(_mrr_-_hsd_RMS-_mcr_BC) Φ80_lac_ZΔM15 Δ_lac_X74 _rec_A1 _ara_D139 Δ(_ara, leu)7697 _gal_U _gal_K _rps_L (StrR) _end_A1 _nup_G

Find the strain and format that fits your needs
DH strains are available in chemically competent and electrocompetent cell formats.
For high-throughput transformation options, choose from our collection of MultiShot formatted comp cells.
Email us for more information on custom formatting options.

사양

제품 유형Chemically Competent Cells

에프에피솜 포함No

플라스미드 품질 개선Yes (endA1)

메틸화 DNA 클로닝Yes (mcrA)

형질전환 효율 수준Medium Efficiency (1 x 108 to 1 x 109 cfu/μg)

항생제 내성 박테리아Yes (Streptomycin)

불안정 DNA 클로닝Not suitable for cloning unstable DNA

블루/화이트 스크리닝Yes (lacZΔM15)

고처리량 호환성High

플라스미드High Copy Plasmid

비메틸화 DNA 준비No

재조합 감소Yes (recA1)

배송 조건Dry Ice

T1 Phage - 저항성(tonA)No

종E. coli (K12)

형식96-well Plate

제품라인MultiShot™

수량5 x 96-well Plate (15 μL/Well)

Unit SizeEach