Thermo Fisher Scientific MultiShot TOP10 Chemically Competent E. coli

Thermo Fisher Scientific MultiShot™ TOP10 Chemically Competent E. coli

Invitrogen™ MultiShot™ TOP10 Chemically Competent E. coli cells are derived from DH10B and are one of the most popular laboratory strains.
Packaged in five 96-well microtiter plates (15 μL aliquots), they simplify high-throughput workflows and provide transformation efficiencies of >1 × 10⁸ cfu/μg control plasmid DNA.
MultiShot TOP10 is a versatile strain suitable for multiple routine cloning applications.

Benefits

• Ideal for high-efficiency cloning and plasmid propagation
• Convenient microtiter plate format for automated, high-throughput cloning
• mcrA mutation supports efficient transfection of methylated DNA
• lacZΔM15 allele permits rapid blue/white screening
• endA1 mutation reduces endonuclease activity and improves plasmid DNA yield
• recA1 mutation minimizes unwanted recombination

High-Throughput Transformation

Designed for use directly in 96-well plates, MultiShot TOP10 Chemically Competent cells enable all transformation steps—up to plating—in the same well, saving time and reducing contamination risk.
After adding DNA, transformation can be performed by heat shock at 42°C using a heat block or thermocycler.
Using TA Cloning and TOPO Cloning protocols, including controls, yields of 100–400 colonies per agar plate can be expected.

Genotype

F⁻ mcrA Δ(mrr-hsdRMS-mcrBC) Φ80lacZΔM15 ΔlacX74 recA1 araD139 Δ(ara, leu)7697 galU galK rpsL (StrR) endA1 nupG

Available Formats

DH strains are available in both chemically competent and electrocompetent forms.
For high-throughput applications, MultiShot formatted competent cells are also available.
For custom formatting options, contact Thermo Fisher Scientific at customorders@thermofisher.com.

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