Thermo Fisher Scientific TNIK Polyclonal Antibody
TNIK 단백질을 인식하는 Thermo Fisher Scientific의 rabbit polyclonal 항체로, IHC(P) 및 ELISA에 사용 가능. 인간 및 다양한 종에 반응하며, 항원 친화 크로마토그래피로 정제됨. 액상 형태로 제공되며 1 mg/mL 농도, 연구용 전용.
- 판매단위
- pk
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Applications
Immunohistochemistry (Paraffin) [IHC (P)]
- Tested Dilution: 5–10 µg/mL
ELISA
- Tested Dilution: Assay-dependent
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Non-human primate, Rabbit, Bat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic 18 amino acid peptide from internal region of human TNIK |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS |
| Contains | 0.1% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2550544 |
Product Specific Information
Percent identity with other species by BLAST analysis:
- 100%: Human, Gorilla, Gibbon, Monkey, Marmoset, Bat, Rabbit
- 94%: Dog, Elephant, Panda, Horse
- 89%: Mouse, Rat
- 83%: Bovine
For IHC(P), use heat-induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen.
Target Information
TNIK (TRAF2 and NCK Interacting Kinase)
TNIK is characterized by an N-terminal kinase domain and a C-terminal GCK domain that serves a regulatory function. It is mainly expressed in the brain, heart, and spleen and acts as a specific effector of RAP2, regulating the actin cytoskeleton. TNIK is autophosphorylated in a lys54-dependent manner within its kinase domain and plays a key role in cytoskeleton regulation. It phosphorylates gelsolin, an actin-severing protein, suggesting its role in cytoskeletal organization.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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