Thermo Fisher Scientific TNIK Polyclonal Antibody

Applications

Immunohistochemistry (Paraffin) [IHC (P)]

• Tested Dilution: 5–10 µg/mL

ELISA

• Tested Dilution: Assay-dependent

Product Specifications

Product Specific Information

Percent identity with other species by BLAST analysis:

• 100%: Human, Gorilla, Gibbon, Monkey, Marmoset, Bat, Rabbit
• 94%: Dog, Elephant, Panda, Horse
• 89%: Mouse, Rat
• 83%: Bovine

For IHC(P), use heat-induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen.

Target Information

TNIK (TRAF2 and NCK Interacting Kinase)
TNIK is characterized by an N-terminal kinase domain and a C-terminal GCK domain that serves a regulatory function. It is mainly expressed in the brain, heart, and spleen and acts as a specific effector of RAP2, regulating the actin cytoskeleton. TNIK is autophosphorylated in a lys54-dependent manner within its kinase domain and plays a key role in cytoskeleton regulation. It phosphorylates gelsolin, an actin-severing protein, suggesting its role in cytoskeletal organization.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.