Thermo Fisher Scientific WNK2 Monoclonal Antibody (2E10)
Thermo Fisher Scientific의 WNK2 Monoclonal Antibody (2E10)는 인간 WNK2 단백질을 인식하는 Mouse IgG1 항체로, WB, IHC(P), ELISA에 적합합니다. Affinity chromatography로 정제되었으며, PBS buffer에 보존제 없이 제공됩니다. 연구용으로만 사용 가능합니다.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1–5 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 3 µg/mL |
| ELISA | 0.1 ng/mL |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 2E10 |
| Immunogen | WNK2 (NP_006639, 2118–2217 a.a.) partial recombinant protein with GST tag (MW of GST tag: 26 kDa) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | See Label |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.4 |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
Sequence of this protein is as follows:
KGTFTDDLHK LVDEWTSKTV GAAQLKPTLN QLKQTQKLQD MEAQAGWAAP GEARAMTAPR AGVGMPRLPP APGPLSTTVI PGAAPTLSVP TPDPESEKPD
Target Information
WNK2 is a serine/threonine kinase involved in signal transduction and cellular communication. WNK kinases contain a lysine upstream of the traditional position, within a glycine string. This lysine functions as an anchor and orients ATP through interactions with the alpha and beta phosphoryl groups. The catalytic domains of WNK2, WNK3, and WNK4 are 95% homologous to WNK1.
The human WNK1 gene encodes a 2,382 amino acid protein primarily expressed in heart, kidney, muscle, and distal nephron. The human WNK3 gene encodes a protein primarily expressed in brain, and WNK4 encodes a 1,243 amino acid protein expressed in kidney.
Aberrant function of WNK kinases and their associated signaling pathways are implicated in hypertension, increased renal salt reabsorption, and impaired K⁺ and H⁺ excretion.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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