Thermo Fisher Scientific E4F1 Polyclonal Antibody
E4F1 단백질을 인식하는 Rabbit Polyclonal 항체로, Western blot 및 Immunoprecipitation에 적합합니다. Human 시료에 반응하며, 항원 친화 크로마토그래피로 정제되었습니다. 세포주기 조절 및 p53/pRB 경로 연구에 활용 가능합니다.
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Applications
Western Blot (WB)
- Tested Dilution: 1:2,000–1:10,000
Immunoprecipitation (IP)
- Tested Dilution: 2–5 µg/mg lysate
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Region between residue 725 and the C-terminus (residue 784) of human E4F1 (p120E4F) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | Tris citrate/phosphate, pH 7–8 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
Product Specific Information
- Recommended shelf life: 1 year from date of receipt
- Based on 100% sequence identity, this antibody is predicted to react with Mouse
Target Information
E4F1 may function as a transcriptional repressor and ubiquitin ligase mediating ubiquitination of chromatin-associated TP53. It plays a role in cell survival and proliferation through control of the cell cycle, functioning in the p53 and pRB tumor suppressor pathways and regulating cyclin CCNA2 transcription.
Identified as a cellular target of the adenoviral oncoprotein E1A, E4F1 is required for both transcriptional activation and repression of viral genes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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