Thermo Fisher Scientific ATG3 Monoclonal Antibody (OTI2C12)
ATG3 단백질을 인식하는 Mouse IgG1 단일클론 항체로, Western blot, IHC, ICC/IF, Flow Cytometry 등 다양한 응용 가능. 인간과 마우스 반응성. 액상 형태, 1 mg/mL 농도, -20°C 보관. 연구용으로만 사용.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:150 |
| Immunocytochemistry (ICC/IF) | 1:50–1:100 |
| Flow Cytometry (Flow) | 1:100 |
Product Specifications
| Item | Description |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI2C12 |
| Immunogen | Full-length human recombinant protein of ATG3 produced in HEK293 cells |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Affinity Chromatography |
| Storage Buffer | PBS, pH 7.3, with 1% BSA, 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2723937 |
Target Information
ATG3 (APG3) is a widely expressed conjugating enzyme essential for APG8 lipidation, a critical step in autophagy initiation. Autophagy enables degradation of cellular proteins via the autophagosomal-lysosomal pathway, supporting normal growth control and nutrient preservation under starvation. Dysregulation of this process may occur in tumor cells. ATG3 acts as an E2-like enzyme forming the Atg8-phosphatidylethanolamine (Atg8-PE) conjugate, crucial for autophagosome formation. The process is negatively regulated by TOR through phosphorylation of autophagy protein APG1.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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