Thermo Fisher Scientific ReproSeq PGS 318 View Kit, without chips

Thermo Fisher Scientific ReproSeq™ PGS 318 View Kit (without chips)

The ReproSeq™ PGS View kits enable a rapid benchtop next-generation sequencing (NGS) workflow for aneuploidy analysis. When used with the Ion PGM™ System, they allow rapid and affordable detection of aneuploidies across all 24 chromosomes (22 autosomes and the X and Y chromosomes), supporting research on pre-implantation embryos.

Using as little as 6 pg of DNA from a single cell, ReproSeq PGS View kits enable accurate detection of whole chromosome and chromosome arm events (≥48 Mb) in as little as 8 hours.

Turnaround time for the ReproSeq PGS View workflow is as little as 8 hours with the Ion 314™ Chip and as little as 10 hours with the Ion 318™ Chip.

Cost-effective analysis workflow with sample throughput flexibility

The ReproSeq PGS View kits and the Ion PGM System deliver scalability and flexibility for various levels of sample throughput. Choose from 3 sequencing chips for processing 2 to 24 samples, minimizing the need for batching and ensuring cost efficiency.

Rapid turnaround for time-sensitive samples

Leveraging the speed of semiconductor sequencing and template preparation by isothermal amplification, the ReproSeq PGS View kits provide a fast benchtop NGS workflow for aneuploidy analysis.
Go from single cells isolated from a pre-implantation embryo to aneuploidy detection in as little as 8 hours (2 samples) or 10 hours (up to 24 samples).

Kit Components

The ReproSeq™ PGS 318 View Kit, without chips, includes all reagents for whole genome amplification, library construction, template preparation, and sequencing using the Ion PGM System for processing a total of 192 samples.

• Ion SingleSeq™ Kit (24 reactions/kit) — extraction, amplification, and barcoding of genomic DNA; completion in <4 hours (including hands-on time)
• Ion PGM™ Template IA 500 Kit (A24622, 8 reactions/kit) — fastest template preparation (~2 hours, including hands-on time); DNA clonally amplified onto bead surface through non-emulsion, isothermal reaction
• Ion PGM™ Hi-Q™ View Sequencing Kit (A30044, 8 reactions/kit at 200 bp) — advanced sequencing chemistry supporting all 200 bp sequencing needs
• Ion PGM™ Wash 2 Bottle Kit (A25591) — conditioning solution and Wash 2 bottle usable for up to 40 runs before replacement

Notes

• Requires Ion OneTouch™ ES, Ion PGM™ Enrichment Beads (4478525), and Ion 318™ Chip Kit v2 BC (4488150) for use.
• Compatible only with the Ion PGM System.

Specifications

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