Gibco™

Expi293™ Expression System Kit

The Expi293 Expression System is a complete solution for rapid, high-yield protein production from mammalian 293 cells. It is based자세히 알아보기

The Expi293 Expression System is a complete solution for rapid, high-yield protein production from mammalian 293 cells. It is based on integrated components including high-density Expi293F cells in Expi293 Expression Medium and the cationic lipid-based ExpiFectamine 293 transfection reagent in combination with specialized transfection enhancers.

The Expi293 Expression System features:
• Up to 1 g/L of protein; 2- to 10-fold higher protein yields than previous generation transient expression systems such as the FreeStyle 293 Expression System
• Rapid production of recombinant proteins in 5–7 days
• High density cultures of Expi293F cells that result in more expressing cells per milliliter of culture
• Native folding and mammalian post-translational modifications of expressed proteins
• Easy, robust culture and transfection protocols
• Scalable expression from <1 mL to >10 L

The components included in the Expi293 Expression System Kit are: two vials of frozen Expi293F cells, one liter of Expi293 Expression Medium, one ExpiFectamine 293 Transfection kit sufficient to transfect 1 liter of culture, Opti-MEM I Reduced Serum Medium, and an antibody-expressing positive control vector. All components of the Expi293 Expression System are also available for individual purchase.

Engineered Expi293F cell lines for a range of applications are also available for individual purchase or as part of kits: Expi293F GnTI- Cells (Cat. No. A39240). Expi293F Inducible Cells (Cat. No. A39241), and Expi293F Inducible GnTI- Cells (Cat. No. A39242).

사양

세포주Expi293F™

배달 유형Transfection

셀 유형293-derived Cells

프로모터CMV

Transfection TechniqueLipid-based Transfection

발현 시스템Mammalian

발현 안정성Transient

용도(애플리케이션)Protein Expression

주요 기능Protein Production, Constitutive Expression

반응 수33

제품라인Expi293™

제품 유형Expression System Kit

단백질 태그Untagged

수량1 kit

시스템 유형Expi293™ Expression System

Unit SizeEach