Thermo Fisher Scientific TLR4 Polyclonal Antibody
TLR4 단백질을 인식하는 Thermo Fisher Scientific의 Rabbit Polyclonal Antibody. 다양한 종(Human, Mouse 등)에 반응하며 WB, IHC, ICC/IF, Flow Cytometry 등 다중 응용 가능. 항원 친화 크로마토그래피로 정제된 고품질 시약으로 면역학 및 병리 연구에 적합.
- 판매단위
- pk
카탈로그
1개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific TLR4 Polyclonal Antibody
Thermo Fisher Scientific TLR4 Polyclonal Antibody
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 2–5 µg/mL | View 4 publications |
| Immunohistochemistry (Paraffin) (IHC (P)) | 5 µg/mL | View 1 publication |
| Immunocytochemistry (ICC/IF) | 2–5 µg/mL | – |
| Flow Cytometry (Flow) | 20 µg × 10⁶ cells | – |
| in situ PLA (PLA) | – | View 1 publication |
Product Specifications
| Item | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rhesus monkey, Rat |
| Published Species | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide corresponding to amino acids 30–80 of mouse TLR4 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C (short term); -20°C (long term), avoid freeze/thaw cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2540651 |
Product Specific Information
Suggested positive control: recombinant protein, RAW, Daudi.
Target Information
TLR4 is a member of the Toll-like receptor (TLR) family, which plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. TLR4 is most abundantly expressed in the placenta and the myelomonocytic subpopulation of leukocytes. Mammalian cells respond to LPS by activating TLR4, which forms part of a multi-protein complex containing CD14, LY96, and TLR4, involved in signal transduction induced by lipopolysaccharides from Gram-negative bacteria.
TLR4 recognizes pathogen-associated molecular patterns (PAMPs) and mediates cytokine production necessary for effective immunity. Mutations in the TLR4 gene are associated with differences in LPS responsiveness. Several transcript variants of the TLR4 gene exist, though most have uncertain protein-coding potential. TLR4 is expressed by peripheral blood monocytes, a small population of B-cells, and in human placenta. Studies using TLR4-deficient mice show increased susceptibility to Gram-negative sepsis, confirming its key role in LPS recognition.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
제품 이미지
Thermo Fisher Scientific 상품 둘러보기
전체보기
문의
0개 · 배송·재고 문의는 실시간 상담이 빠릅니다아직 등록된 문의가 없어요.