Thermo Fisher Scientific ATP synthase Monoclonal Antibody (12F4AD8AF8)

Applications

Tested Dilution

Publications

Immunocytochemistry (ICC/IF)

5 µg/mL

-

Flow Cytometry (Flow)

1 µg/ 10^6 cells

-

Product Specifications

Species Reactivity

Bovine, Human, Mouse

Host/Isotype

Mouse / IgG2b, kappa

Class

Monoclonal

Type

Antibody

Clone

12F4AD8AF8

Immunogen

Bovine Complex V

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Ammonium sulfate precipitation

Storage buffer

HEPES buffered saline

Contains

0.02% sodium azide

Storage conditions

4° C, do not freeze

Shipping conditions

Wet ice

RRID

AB_2533547

Product Specific Information

Positive control: Bovine heart mitochondria

Target Information

Complex V, also called F1F0ATPase or ATP synthase, is responsible for ATP production in oxidative phosphorylation and can work in reverse as a proton pumping ATPase. The enzyme was thought to be localized exclusively to mitochondria. However, it has recently been identified on the plasma membrane of several cell types including hepatocytes where it functions as the HDL receptor, on endothelial cells where it may act as the angiostatin receptor, and on the surface of cancer cells. The enzyme in mammals is composed of 17 subunits, five of which make up the easily detached F1. The remainder subunits are components of two stalk domains and the proton pumping F0 part of the machinery. Two of the subunits of the F0 part are encoded on mitochondrial DNA while the other subunits are nuclear encoded. Mutations in the mitochondrial-encoded subunits of ATP synthase (Complex V) cause OXPHOS disease.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.