Thermo Scientific™

FaqI (BsmFI) (2 U/μL)

5G G G A C N10↓ 33C C C T G N14↑ 5Thermo Scientific FaqI (BsmFI) restriction enzyme자세히 알아보기

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5`			G	G	G	A	C	N10	↓		3`
3`			C	C	C	T	G	N14	↑		5`

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Thermo Scientific FaqI (BsmFI) restriction enzyme recognizes GGGAC(10/14)^ sites and cuts best at 37°C in Tango (+SAM) buffer (isoschizomers: BslFI, BsmFI). See Reaction Conditions for Restriction Enzymes for a table of enzyme activity, conditions for double digestion, and heat inactivation for this and other restriction enzymes. Note: Also available as a FastDigest enzyme for rapid DNA digestion.

Thermo Scientific conventional restriction endonucleases are a large collection of high quality restriction enzymes, optimized to work in one of the buffers of the Five Buffer System. In addition, the universal Tango buffer is provided for convenience in double digestions. All of the enzymes exhibit 100% activity in the recommended buffer and reaction conditions. To ensure consistent performance, Thermo Scientific restriction enzyme reaction buffers contain premixed BSA, which enhances the stability of many enzymes and binds contaminants that may be present in DNA preparations.

Features

• Superior quality—stringent quality control and industry leading manufacturing process
• Convenient color-coded Five Buffer System
• Includes universal Tango buffer for double-digestions
• BSA premixed in reaction buffers
• Wide selection of restriction endonuclease specificities

Applications

• Molecular cloning
• Restriction site mapping
• Genotyping
• Southern blotting
• Restriction fragment length polymorphism (RFLP)
• SNP

Note: FaqI requires only Mg2+ for its activity, but is stimulated by S-adenosylmethionine. 0.05mM S-adenosylmethionine gives more than a 2-fold increase in FaqI activity. Still, complete cleavage of some substrates is difficult to achieve. FaqI concentration is determined by the maximum cleavage level achieved when no change in the fragmentation pattern is observed with further enzyme increase. FaqI may remain associated with the cleaved DNA. This may cause DNA band shifting during electrophoresis. To avoid atypical DNA band patterns, use the 6X DNA Loading Dye & SDS Solution for sample preparation or heat the digested DNA in the presence of SDS prior to electrophoresis. For methylation sensitivity, refer to product specifications.

사양

농도2 U/μL

메틸화 민감도CpG Methylation-Sensitive, Not Dam Methylation-Sensitive, Not Dcm Methylation-Sensitive

효소FaqI (BsmFI)

함께 사용가능한 버퍼10X Buffer Tango

열 불활성화에 대한 민감성Yes

최적 반응 온도37°C

Type IIS REYes

수량100 units

제품 유형Restriction Enzyme

연구 카테고리Scarless Cloning

Unit SizeEach