Thermo Fisher Scientific Phospho-HEC1 (Ser76, Ser77) Polyclonal Antibody
인간 HEC1 단백질의 Ser76, Ser77 인산화 부위를 인식하는 Rabbit Polyclonal Antibody. Western blot, ICC/IF, ELISA에 사용 가능. 항원 친화 크로마토그래피로 정제되었으며, PBS 용액 형태로 제공. 연구용으로 세포 분열 스핀들 체크포인트 연구에 적합.
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Thermo Fisher Scientific Phospho-HEC1 (Ser76, Ser77) Polyclonal Antibody
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:300 |
| Immunocytochemistry (ICC/IF) | 1:150 |
| ELISA | Assay-dependent |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic phosphopeptide corresponding to the amino acids around Ser76/77 conjugated to KLH |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage buffer | PBS |
| Contains | No preservative |
| Storage conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2552327 |
Product Specific Information
- Recommended positive controls: HeLa, Blocking peptide (GTX70015-PEP)
- Store product as a concentrated solution.
- Centrifuge briefly prior to opening the vial.
Target Information
Hec1 is one of several proteins involved in spindle checkpoint signaling. This surveillance mechanism assures correct segregation of chromosomes during cell division by detecting unaligned chromosomes and causing prometaphase arrest until the proper bipolar attachment of chromosomes is achieved.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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