Thermo Fisher Scientific Phospho-HEC1 (Ser76, Ser77) Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:300

-

Immunocytochemistry (ICC/IF)

1:150

-

ELISA (ELISA)

Assay-dependent

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic phosphopeptide corresponding to the amino acids around Ser 76/77 conjugated to KLH if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:HEC1,uniProtId:O14777-1,ncbiNodeId:9606,antigenRange:77,antigenLength:642,antigenImageFileName:PA5-34979_HEC1_O14777-1_Rabbit.svg,antigenImageFileNamePDP:PA5-34979_HEC1_O14777-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS

Contains

no preservative

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2552327

Product Specific Information

Recommended positive controls: HeLa, Blocking peptide (GTX70015-PEP).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

Hec1 is one of several proteins involved in spindle checkpoint signaling. This surveillance mechanism assures correct segregation of chromosomes during cell division by detecting unaligned chromosomes and causing prometaphase arrest until the proper bipolar attachment of chromosomes is achieved.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.