Himedia Collagenase Type I Solution, 0.25% w/ 0.25% Collagenase type I in PBS and 20% Fetal Bovine Serum

Collagenase Type I Solution (0.25% in PBS with 20% Fetal Bovine Serum)

Collagenases have specificity for the sequence R-Pro-(-X-Gly-Pro) where X is most often a neutral amino acid.
Bacterial collagenases have a broader specificity than vertebrate collagenases, allowing them to degrade both water-insoluble native collagens and water-soluble denatured collagens.

The extracellular matrix (ECM) in animal tissue is a complex mixture of collagens, glycoproteins, and proteoglycans. Effective breakdown of this matrix is essential to isolate single cells without altering cellular structures.

Crude collagenase is a mixture of collagenases and other proteolytic enzymes such as clostripain, tryptic, and neutral protease activities.
This combination of collagenolytic and proteolytic activities efficiently breaks down intercellular matrices, which is crucial for tissue dissociation.

Collagenases are endopeptidases derived from the fermentation of Clostridium histolyticum that digest native collagen in the triple helix region.

TCL116 is a 0.25% Collagenase Type I solution in PBS containing 20% Fetal Bovine Serum.

제품 사양