Thermo Fisher Scientific PRKAR1B Monoclonal Antibody (OTI2A3), TrueMAB
PRKAR1B 단백질을 인식하는 Mouse IgG1 단일클론 항체로 Western blot, IHC, ICC/IF, Flow Cytometry에 적합. HEK293T에서 생산된 full-length human recombinant protein을 면역원으로 사용. Lyophilized 형태로 제공되며, 고순도 친화 크로마토그래피 정제. 연구용 전용.
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Thermo Fisher Scientific PRKAR1B Monoclonal Antibody (OTI2A3), TrueMAB
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:150 |
| Immunocytochemistry (ICC/IF) | 1:100 |
| Flow Cytometry (Flow) | 1:100 |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI2A3 |
| Immunogen | Full length human recombinant protein of human PRKAR1B produced in HEK293T cell |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).
Target Information
The second messenger cyclic AMP (cAMP) mediates diverse cellular responses such as proliferation, ion transport, metabolism regulation, and gene transcription through activation of cAMP-dependent protein kinase (PKA).
Activation occurs when cAMP binds to the regulatory subunits of the PKA holoenzyme, releasing active catalytic subunits. Elevated cAMP levels lead to nuclear translocation of PKA, phosphorylation of CREB on serine 133, and initiation of transcription via TFIIB binding to TBP1, linking phospho-CREB to the RNA polymerase II complex.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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