Thermo Fisher Scientific YBX1 Monoclonal Antibody (4C7)
YBX1 단백질을 인식하는 마우스 단일클론 항체로, Western blot, ICC/IF, ELISA에 적합합니다. 인간 시료에 반응하며, PBS 버퍼에 보존된 액상 형태입니다. 연구용으로만 사용되며, DNA 수리 및 전사 조절 연구에 유용합니다.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1–5 µg/mL |
| Immunocytochemistry (ICC/IF) | 10 µg/mL |
| ELISA | 0.1 ng/mL |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2a, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 4C7 |
| Immunogen | YBX1 (NP_004550, amino acids 51–139) partial recombinant protein with GST tag (MW of GST tag alone: 26 kDa) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | See Label |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.4 |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
Sequence of this protein is as follows:
DKKVIATKVL GTVKWFNVRN GYGFINRNDT KEDVFVHQTA IKKNNPRKYL RSVGDGETVE FDVVEGEKGA EAANVTGPGG VPVQGSKYA
Target Information
YBX1 binds to splice sites in pre-mRNA and regulates splice site selection. It stabilizes cytoplasmic mRNA and modulates translation by influencing the interaction between mRNA and eukaryotic initiation factors. YBX1 binds to promoters containing a Y-box (5'-CTGATTGGCCAA-3'), such as HLA class II genes, and regulates transcription of numerous genes. It promotes separation of DNA strands with mismatches or cisplatin modifications, exhibits endonucleolytic activity, and may contribute to DNA repair.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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