Thermo Fisher Scientific RFP-tag Polyclonal Antibody

Applications and Tested Dilutions

Product Specifications

Product Specific Information

• Lyophilized powder should be reconstituted with deionized water (or equivalent) to an antibody concentration of 0.5 mg/mL.
• Well-characterized antibodies against short-sequence epitope tags are widely used in protein expression studies.
• RFP-tags consist of a 15-residue peptide (VNGHEFEIEGEGEGR) derived from amino acids 22–36 of red fluorescent protein (RFP) from Discosoma sea anemone.

Target Information

Red fluorescent protein (TagRFP), derived from Entacmaea quadricolor eqFP611 (Uniprot ID: Q8ISF8), is a valuable tool in biotechnology and cell biology. It serves as a spectrally distinct companion or substitute for green fluorescent protein (GFP) from Aequorea jellyfish.
Key characteristics include:

• Pigment protein with stable emission across pH 4–10
• Maturation via a green intermediate, completed within 12 hours
• Recombinant expression at high temperatures may induce oligomerization
• Abs(max): 559 nm, secondary absorbance at 525 nm
• Strong fluorescence emission peak at 611 nm

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.