Thermo Fisher Scientific TaqMan miRNA ABC Purification Kit - Human Panel A

Applied Biosystems™ TaqMan™ miRNA ABC Purification Kit - Human Panel A

Use the TaqMan™ miRNA ABC Purification Kit to purify high-quality miRNA from blood-related or other difficult sample types without hazardous chemicals.

• Simple and fast workflow using oligonucleotide-conjugated Dynal™ magnetic beads
• Small sample input: 10 μL for blood samples to 50 μL for serum and plasma samples
• RT-PCR inhibitors commonly found in blood-related samples are easily washed away
• No need for hazardous chemicals such as phenol, chloroform, Trizol™ reagent, or guanidine isothiocyanate

Simple and Fast miRNA Purification

The TaqMan™ miRNA ABC (anti-miRNA bead capture) purification kit contains buffers and magnetic beads for single-tube isolation of specific microRNA (miRNA) from small inputs of all human sample types, including blood, serum, plasma, FFPE samples, solid tissues, cultured cells, and saliva in approximately 75 minutes.

Human Panel A and Human Panel B

The TaqMan™ miRNA ABC Purification Kit is offered in two configurations: Human Panel A and Human Panel B.
Each kit includes superparamagnetic Dynabeads™ covalently bound to a unique set of 377 anti-miRNA oligonucleotides corresponding to the assays in the TaqMan™ Array Human MicroRNA A and B cards.
The miRNA isolation process relies on hybridization of miRNAs to the corresponding anti-miRNA oligonucleotides attached to the beads.
Complete lists of miRNAs isolated by the panels can be found in the TaqMan™ miRNA ABC Purification Kit protocol.

miRNA Analysis

The miRNAs isolated using the TaqMan™ miRNA ABC Purification Kit are ready for conversion into cDNA and downstream analysis by quantitative PCR using either single tube (individual) TaqMan™ microRNA assays or the TaqMan™ Array Human microRNA A and B cards.
Example data from various sample types can be obtained using the kit.

For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.

사양 (Specifications)

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