Thermo Fisher Scientific EpCAM Monoclonal Antibody (7E11)
인간 EpCAM 단백질을 인식하는 Mouse IgG1 단클론 항체. Western blot, IHC, ICC, ELISA 등 다양한 응용에 적합. 비결합형 액상 형태로 제공되며, 장기 보관 시 -20°C에서 냉동 보관 권장. 연구용으로만 사용 가능.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (IHC) | - |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:200–1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:100 |
| ELISA | 1:10,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Published Species | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 7E11 |
| Immunogen | Purified recombinant fragment of human EPCAM expressed in E. coli |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | Not Determined |
| Storage Buffer | Ascites |
| Contains | 0.03% Sodium Azide |
| Storage Conditions | Store at 4°C for short term. For long term, store at -20°C avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet Ice |
| RRID | AB_11000092 |
Product Specific Information
MA5-15640 targets EpCAM in indirect ELISA, IHC, and WB applications, showing reactivity with human samples.
The immunogen is a purified recombinant fragment of human EpCAM expressed in E. coli.
MA5-15640 detects EpCAM with a predicted molecular weight of approximately 35 kDa.
Target Information
Ep-CAM (epithelial adhesion molecule, epithelial specific antigen, ESA) is a transmembrane glycoprotein (~40 kDa) expressed in epithelial tissues. It functions as a calcium-independent cell adhesion molecule and influences cell cycle, proliferation, and metabolism through induction of proto-oncogene c-myc and cell cycle regulators cyclin A and E.
Ep-CAM-mediated adhesions negatively regulate cadherin-mediated adhesions, affecting epithelial differentiation and growth. Overexpression is associated with enhanced epithelial proliferation and is a marker for carcinomas of epithelial origin.
Ep-CAM is expressed on the basolateral surface of most simple epithelia and many carcinoma types, distinguishing adenocarcinomas from pleural mesotheliomas.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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